碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 107 / Thraustochytrids is a kind of heterotrophic microalgae. The ratio of lipid to cell dry weight can reach 50%. It is considered to have the potential to develop the biofuel. In some species, even high valued compounds such as carotenoids are produced. This study selected Aurantiochytrium sp. AP45 as the optimal condition for the production of astaxanthin and life cycle assessment. During the screening stage of the medium, the results showed that the medium with high nitrogen content favored the accumulation of DHA. At the same carbon source concentration and the C/N ratio, the medium with low nitrogen content was more favorable for the accumulation of astaxanthin.
Fermentation culture results showed that Aurantiochytrium sp. AP45 is not suitable for the cultivation of waste cooking oil as a carbon source. In addition, there is a significant difference in the accumulation of astaxanthin with or without LED blue light at Fed-batch cultivation. Aurantiochytrium sp. AP45 without LED blue light, the accumulation of astaxanthin per unit algae is 7.27 g/g, and the total yield of astaxanthin is 62.17 g/L. If it is with LED blue light during fermentation, the accumulation of astaxanthin per unit algae can reach 28.20 g/g, and the total output of astaxanthin is 172.06 g/L, which is about 4 times higher.
The life cycle assessment of carbon footprint and environmental impact was performed on the results of the astaxanthin cultured in Aurantiochytrium sp. AP45. The functional unit was 1 g astaxanthin. The cultivation stage is a great emission source of carbon footprint and environmental impact. The carbon footprint of the cultivation stage is about 2,180 kgCO2e/g, mainly from the electricity required for LED illumination. The acetone used in the extraction stage is a great impact source at Carcinogens category .
Most of the existing research on micro-algae put a lot of effort on algae biofuels. However, there are very few studies on health foods and cosmetics. The life cycle assessment of health foods for heterotrophic microalgae is almost none. Therefore, the present investigation contributes to the life cycle assessment study of the astaxanthin production in heterotrophic algae.
| Identifer | oai:union.ndltd.org:TW/107NSYS5277009 |
| Date | January 2019 |
| Creators | Ting-Yu Kuo, 郭庭妤 |
| Contributors | Tse-Min Lee, 李澤民 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 89 |
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