碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 107 / Thraustochytrids are marine unicellular protist. They are similar to the common heterotrophic bacteria, which relies on the decomposition of organic matter from other organisms. They show absolute demand for sodium, widely distributed in mangroves, sea areas or estuaries. They can produce some high-priced compounds, such as astaxanthin. In this study, the feasibility of algae for production of astaxanthin was to screen the algae strain with the highest astaxanthin content, and the astaxanthin production was increased by using different culture conditions and waste cooking oil. The results showed that 25 strains of Thraustochytrids were isolated from mangroves. Among them, 14 strains were known as Thraustochytrids, 1 strain was yeast, and the remaining 10 strains had not been determined. The strain YA7.1, who was selected from Kaohsiung Yong-an mangrove and showed the highest astaxanthin production, was used in the following experiments. In the experiment of optimizing the medium, the carbon source was glucose or waste cooking oil, and the nitrogen source was tryptone and yeast extract. The best astaxanthin yields for glucose and waste cooking oil were 197 μg/L and 70 μg/L, respectively using 125 ml flask culture system. Then, by using the fermenter culture, the best astaxanthin productions were 320 μg/L and 308 μg/L for glucose and waste cooking oil, respectively. After the blue LED illumination using glucose as the carbon source, the production of astaxanthin was twice that of the unilluminated control, but the use of waste cooking oil as the carbon source, the production of astaxanthin was 10 times lower. Based on the above results, it is feasible to use waste cooking oil as a carbon source to produce astaxanthin, but the increase in the production of astaxanthin by blue LED illumination was only workable using glucose as the carbon source.
| Identifer | oai:union.ndltd.org:TW/107NSYS5277013 |
| Date | January 2019 |
| Creators | Liang-Yu Tsai, 蔡喨宇 |
| Contributors | Hsiu-Chin Lin, Tse-Min Lee, 林秀瑾, 李澤民 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 59 |
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