博士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 107 / Gingival recession (GR) has been defined as the loss of soft tissue covering the roots. This condition can cause the roots to be directly exposed to oral microenvironment, causing problems such as dental caries and extreme tooth sensitivity. Even though many pathogenic factors have been reported, the exact mechanism of gingival atrophy has not been clarified so far, and there is a lack of safe and effective therapies. Taiwan is rich in marine biodiversity and a treasure trove of drug development. In the present study, marine-derived substance was first used to screen cell viability for human gingival fibroblast (hGF-1) and oral mucosa fibroblast (OMF). In the experiment, it was found that the crinoid-derived compound had an effect promote on the proliferation and cell viability of the gingival tissue cells, but the oral mucosal fibroblasts had no effect. Our results showed that crinoid compound can significantly improve cell migration and wound healing of hGF-1 cells. However, it had no effect on OMF cells. The action of this crinoid compound may be affected by focal adhesion kinase (FAK), F-actin, and fibronectin. Moreover, crinoid compound increases the oxidative phosphorylation (OXPHOS) performed in mitochondria and glycolytic effects in the cytoplasm, enhances mitochondrial function, and produce the energy required for ATP to provide cell migration. Therefore, the crinoid compound can promote gingival regeneration and is a promising therapeutic agent for GR. It may also be applied to the development of future oral health antibacterial mouthwashes, clinical drugs or dental dressings in the future. At the same time, it is also more conducive to industry-university cooperation and further solve the problem of patients with gingival recession.
| Identifer | oai:union.ndltd.org:TW/107NSYS5277016 |
| Date | January 2019 |
| Creators | Chung-Chih Tseng, 曾崇智 |
| Contributors | Zhi-Hong Wen, 溫志宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 122 |
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