碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 107 / This study aims to investigate the optimal growth conditions for the larval culture of Caridina brevicarpalis so that the informative results can be used as background knowledge for seed production as well as population reservation purpose. The first three experiments were conducted to investigate the effects of salinity, microalgae species and microalgae densities on the metamorphic larval survival rate and growth rate (larval stage index, LSI) in the end of each larval culture experiment. The last experiment was involved in the comparison between the two progeny sources of wild and artificially captivatedly induced spawners on their own respective larval survivals. Constant conditional setups for larval density (25 ind./L), photoperiod (12L:12D) and temperature (25±1℃) were kept all the same throughout each and every experiment described above.
It was found that the highest metamorphic larval survival rate occurred at salinity level of 15‰ (53.3%) which was significantly higher than those cultivated at 20‰ (33.3%), 25‰ (19.3%), 10‰ (18.0%), and 30‰ (0%) being the lowest.
The highest metamorphic larval survival rate occurred in the group of larvae received Tetraselmis. chui (62.7%) which was significantly higher than those of the rest three treatments, Thalassiosira weissflogii (4.0%) Chaetoceros muelleri (0.7%) Isochrysis galbana (0%).
Of the four feeding densities of T.chui (0.5, 1.0, 2.0, 4.0) ×105 cells/ml, significant higher metamorphic larval survival rate appeared in those larvae received density either at 0.5×105cells/ml (57.3%) or 1.0×105 cells/ml (46.0%) than the other two densities at 2.0×105cells/ml (27.3%) and 4.0×105 cells/ml (0%).
At salinity level of 15 ‰ and feeding density of T. chui (0.5×105 cells/ml), metamorphic larval survival rate of the artificially produced progenies (64.0%) was higher than that of the wild origin (51.3%).
It is suggested that the optimum salinity of 15 ‰ and microalgal feeding densities of 0.5-1.0×105cells/ml are suitable for larval culture of C. brevicarpalis under the conditional photoperiod 12L: 12D and water temperature 25±1℃. And Larval stages of C. brevicarpalis is divided into ten stages (Zoea stage I-V, Mysis stage I-IV, Post Larva) within 24 days of culture.
| Identifer | oai:union.ndltd.org:TW/107NTOU5086031 |
| Date | January 2019 |
| Creators | Lin, Chia-Chi, 林家齊 |
| Contributors | Huang, Yii-Shing, 黃沂訓 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 53 |
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