碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 107 / This study aims to investigate the optimal growth conditions for the larvae culture of Dwarf-bamboo shrimp Atyopsis spinipes in order to provide criteria for artificial propagation for future development of ornamental shrimp industry. There were three factors of salinity, microalgal species and microalgal feeding density separately involved in the first three experiments to investigate each of the respective best condition on the larval survival and growth. Saline reduction acclimation on the metamorphic post-larval survival was further conducted to complete the study series as a whole. Additional information on larval developmental stages from Zoea I to Post larvae stage were documented and discussed in the text.
The highest larval metamorphic survival rate was found at salinity level of 25‰ (60.8%) which was significantly higher than those at 30‰ (28.9 %), 20 ‰ (16.6 %) ,15 ‰ (31.7 %), and 10‰ (0.0 %) after each of the triplicated five levels of salinity treatments received microalgae Tetraselmis chui a density of 2x105 cells/ml once every two days. Larval growth (Avg. post-larval final length) showed that there existed no significant differences among the three groups at 30 ‰ (7.95 mm), 25 ‰ (7.76 mm), 20 ‰ (7.78 mm) which were significantly higher than the group of larvae stayed at 15 ‰ (7.51 mm). There’s no growth data available for the group of larvae at salinity level of 10‰ with zero percentage of metamorphic survival.
The highest metamorphic larval survival rate was found in the group of larvae fed T. chui (85.8 %), significantly higher than the group received Chaetoceros muelleri (65.8 %), whilst there existed no final post-larvae survivals both in the groups of larvae fed I. galbana and Thalassiosira Weissflogii after tested larvae receive equal density (2×105cell/ml) of the microalgae of the four species.
As for feeding density trial, the five levels (0.5 ,1, 2, 3, 4) × 10 5cell/ml of T. chui fed to the newly released larvae (Zoea I) found that the highest survival rate (75.0 %) was observed in the group at a density of 2× 105 cell/ml which was significant higher than those at 1× 105 cell/ml (39.2%) followed by 3× 105 cell/ml (26.7 %), 4× 105 cell/ml (19.2 %) and 5× 104 cell/ml(0%), (P< 0.05).
The salinity reduction acclimation experiment was executed to investigate the survival rate of the metamorphic larvae from a previous salinity setup of 25‰ toward a freshwater environment involved in two daily salinity reduction rates of 2 ‰, 5 ‰ plus one with immediate transfer from current salinity level to a complete freshwater environment. Significant higher larval survival rates of the two daily salinity reduction groups, 2 ‰ (100 %) and 5 ‰ (95.0 %), than the immediate transfer group (8.3 %) (P< 0.05) had led us to suggest a 5 ‰ daily salinity reduction rate for a successful acclimation of metamorphic larva from saline back to freshwater environment.
It is concluded that within a constant temperature range of 26±1 ℃, the optimum larval conditional cultural setup for Dwarf-bamboo shrimp, Atyopsis spinipes is a salinity at 25 ‰, a feeding density of T. chui at 2×105 cells/ml and a suitable daily salinity reduction rate of 5 ‰ for post-larval freshwater acclimation.
Key words：Atyopsis spinipes, Larval culture, Salinity, Microalgae, Mircroalgal feeding density, Freshwater acclimation
| Identifer | oai:union.ndltd.org:TW/107NTOU5086032 |
| Date | January 2019 |
| Creators | Kao, Ming-Hung, 高銘鴻 |
| Contributors | Huang, Yii-Shing, 黃沂訓 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 52 |
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