碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 107 / The crystal shrimp (Caridina cantonensis) is widely welcomed by the aquarium market for its bright body color and varied stripes, and it is an important economic species in the aquarium industry in Taiwan. However, there were only few studies on the color regulation and metabolic mechanism of ornamental shrimp in the past, and the cause of color development still needs further investigation. In today's business model, breeders continue to follow the path of traditional breeding methods, and upgrade the sub-marker-assisted selection and systematic breeding management. Therefore, whether it is in academy or industry, the molecular-assisted breeding of crystal shrimp has great potential.This study constructed a transcriptome database of four crystal shrimp strains, including Pure Crystal-Red Shrimp (PR), Pure Crystal-Black Shrimp (PB), and Crystal Red-Golden Shrimp (RG) and Golden Bee Shrimp (GS) pure line and other four lines. By comparing red-crystal shrimp strain, black-crystal shrimp strain and red-gold-crystal shrimp strain three groups of transcriptomes, there were 82,471 differentially expressed Unigenes, of which 12,125 Unigenes were annotated into six databases and had microsatellite sequences.By comparing the differentially expressed genes of PR with PB (PR/PB) and RG with PB (RG/PB) two groups, the microsatellite markers were screened by differential expression, and 13 microsatellite primers were designed. By referring researches of color-related gene, the microsatellite markers were screened for the annotation of the transcripts, and 17 microsatellite primers were designed. Furthermore, microsatellite genotype analysis was performed using a total of 6 sets of crystal shrimp from red crystal (n = 75) and black crystal (n = 75) from three different sources. The frequency of the gene with 9 groups of markers showed significant difference between red crystal and black crystal groups (p <0.05), 4 groups were microsatellite markers for differential expression screening, and 5 groups were labeled Ug40579, Ug40269, Ug2427, CL6891_2, Ug19742. Related to the color-related genes APOD, CYP450 and ABCG.The functional microsatellite molecular markers developed in this study will help the breeding of high quality crystal shrimp seedlings, and also help breeders to develop high-grade commercial shrimp species and new strains, and to study the color development of crustaceans. Provide an important reference.
| Identifer | oai:union.ndltd.org:TW/107NTOU5086039 |
| Date | January 2019 |
| Creators | Wu, Yu-Fang, 伍宇芳 |
| Contributors | Huang, Chang-Wen, 黃章文 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 87 |
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