碩士 / 國立臺灣海洋大學 / 食品科學系 / 107 / Abstract
Colorectal cancer was the highest occurrence in the 2016 cancer incidence rate which was reported by the Ministry of Health and Welfare, Taiwan. Recent studies indicated that an excessive reactive oxygen species (ROS) transforms oxidative stress and inflammation. oxidative stress activates Oxidative stress activates cyclooxygenase (COX-2) and nuclear factor-kappa B (NF-κB), resulting in the abnormal pro- inflammatory cytokines secretion, thereby promoting tumor growth. It has been reported that sulfated polysaccharides of fucoidan from brown algae has anti-oxidative, anti-inflammatory, anti-cancer effects and proved in vivo and in vitro in different types of cancer. In this study, we investigated into the protection of Sargassum hemiphyllum water extract (SHw) in the colorectal cancer. First, the main components of SHw measured to the sulfate and fucoidan contents of 61.9 ± 0.16 and 439.6 mg/g, respectively. In the cell-line experiment, the SHw was toxic to CT26.WT; SHw inhibited the production of NO in LPS-induced macrophage RAW 264.7. We also evaluated the effects of SHw on CT26.WT-bearing mouse and Azoxymethane (AOM)/Dextran sodium sulfate (DSS) induced colorectal cancer as an animal model. The SHw effectively improved the number and volume of tumors, as well as reduced ROS and pro-inflammatory cytokines. Therefore, downstream factors, i.e. hypoxia- inducible factor 1-alpha (HIF-1α) and vascular endothelial growth factor (VEGF) were down regulated by means of decreased ROS and COX-2, thus improving angiogenesis. Finally, SHw was observed to improve the intestinal crypt in tissue sections. In summary, our results revealed that SHw is able to enhance anticancer activity.
| Identifer | oai:union.ndltd.org:TW/107NTOU5253035 |
| Date | January 2019 |
| Creators | Lin, En-Ju, 林恩如 |
| Contributors | Wu, Cheng-Jer, 吳彰哲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 73 |
Page generated in 0.0013 seconds