碩士 / 國立臺灣海洋大學 / 食品科學系 / 107 / Spear shrimp is mainly distributed in India to the Western Pacific coastal area. Spear shrimp is mainly caught on the western coast in Taiwan. The largest producing area is located in Keelung, Taiwan. The shrimp has pink flesh. It has a sharp spike on the head, and the spear shrimp tastes fresh and sweet. It is a common cooking material. According to the literature, the spear shrimp is rich in nutrients with high protein and a large amount of amino acids. The experiment results showed that the crude protein was 22.95%. After lyophilizing, the crude protein of shrimp meat can be as high as 80.87%, and the freeze-dried shrimp meat was crushed and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The SDS-PAGE analysis indicated that the protein expression of shrimp had a number of distinct protein bands between 324 kDa and 10 kDa. The significant protein bands were excised for in-gel digestion and peptide extraction. Protein identification was accomplished by proteomics techniques. Spear shrimp contained proteins such as myosin, actin, troponin, and vitellogenin. The identified proteins were subjected to potential active peptide analysis and simulated enzyme hydrolysis with BIOPEP-UWM database. The results showed shrimp meat may have anti-hypertension, blood sugar lowering, anti-oxidation, anti-thrombosis and other active peptides, among which the probability of lowering blood sugar and anti-hypertension was higher. Simulated hydrolysis of enzymes by 30 different enzymes showed that pepsin had the highest number of active peptides with pH >2, followed by calpain 2 and stem bromelain, and the most abundant peptides were dipeptidyl peptidase IV Inhibitor (DPP IV inhibitor; hypoglycemic peptide), followed by ACE inhibitor which is an antihypertensive peptide. Based on the experimental results, spear shrimp has the potential of lowering blood sugar and antihypertensive activities.
| Identifer | oai:union.ndltd.org:TW/107NTOU5253072 |
| Date | January 2019 |
| Creators | Chen, Hui-Mei, 陳惠美 |
| Contributors | Chang,Yu-Wei, 張祐維 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 68 |
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