碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 107 / Uroteuthis edulis (7.5-16.0 cm), wild Sepia pharaonis (11.5-32.2 cm) and aquaculture Sepia pharaonis (8.3-17.0 cm) were the experience sampled. I used carbon and nitrogen stable, isotope ratios, total arsenic analysis, and inorganic arsenic isolation to investigate the isotope and arsenic content in tissues. I discussed the relationship between cephalopods and arsenic content in different body lengths and environment, and the distribution of inorganic arsenic in tissues to different species.
Two methods were used to analyze total arsenic content, including graphite furnace atomic absorption spectrometry and hydride generation flame atomic absorption spectrometry. I took arsenic analysis of the liver, gill, muscle, stomach and shell of the three individuals. It was found that in addition to the aquaculture sepia and squid in the stomach, the total arsenic content and body length of other tissues showed the significant regression (P < 0.05). I took the same type of body length randomly and compared it, which was found that the total arsenic content of the wild sepia in all tissues. Wild sepias contain the highest total arsenic content. On the other hand, total arsenic content of squids are higher than aquaculture sepias.
In the stable isotope analysis, the δ13C and δ15N of squid and sepia were significantly different (p < 0.05), and there was a significant difference in δ13C and δ15N between wild and aquaculture sepia (p < 0.05).There was a significant positive correlation between δ13C and muscle total arsenic content in the sepia (p < 0.05).There was a significant positive correlation between δ15N and muscle total arsenic content in the squid (p < 0.05).I used of δ15N calculated the trophic level. The consequence showed that aquaculture Sepia pharaonis is the highest (4.62). Wild Sepia pharaonis (4.30) is next to it, and the Uroteuthis edulis (3.81) is the last. Trophic levels are affected by size and species.
The content of inorganic arsenic in the muscle of the U. edulis, wild S. pharaonis and aquaculture S. pharaonis were 0.89, 0.61 and 0.58 μg/g respectively. The ratio of inorganic arsenic to total arsenic in muscle was less than 10%, and was not found to be correlated with body length(p > 0.05).
| Identifer | oai:union.ndltd.org:TW/107NTOU5451001 |
| Date | January 2019 |
| Creators | Chen, Jen-Ho, 陳人禾 |
| Contributors | 鄭學淵 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 59 |
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