碩士 / 國立臺灣海洋大學 / 輪機工程學系 / 107 / Microalgae have high oil content. The process of cultivating microalgae can help maintain the biological system of aquatic cycle, while reduce the impact of land use, food competition and seasonality. In order to improve the stability of the algae cultivation process, the fungus and the microalgae are mixed to adjust the growth conditions of the metabolites and the medium composition in the water, so as to promote the propagation of the algae and rapidly increase the oil content. Therefore, it is necessary to develop a culture technique for reducing the production cost of lipid. In this study, Chlorella vulgaris was selected as the test algae, and symbiotic with Antrodia camphorate. The growth curves of microalgae and fungi, biomass, chlorophyll, particle size, pH, fungal polysaccharides, and lipid contents, etc. were analyzed in order to establish the mutual growth relationship and synergy between the two microorganisms, thereby to increase the production rate of algae and fungi, and reduce the production cost of raw oils and fats. The changes in the properties of algae and fungus under different mixing ratios and cultured days were observed. The experimental results showed that when the Chlorella vulgaris was co-cultured with 5% Antrodia camphorate on the fifth day, the biomass was the highest, which was 0.687 mg/L; chlorophyll was grown by 5.6 times when Chlorella vulgaris was co-cultured with 5% Antrodia camphorate on the fifth day; and the pH value in the culturing water was the lowest (4.11) when Chlorella vulgaris and Antrodia camphorata were co-cultured on the fifth day. The amount of polysaccharide was grown by 1.66 times when Chlorella vulgaris and 15% Antrodia camphorate were co-cultured on the fifth day. In addition, the crude lipid highest of the two microorganisms was the highest (19.61 wt. %) when Chlorella vulgaris was co-cultured with 5% Antrodia camphorate on the fifth day. The heating value of the lipid content reached the highest (17.34 MJ/kg) on the fifth day when the mixing ratio of Antrodia camphora was 15%. The carbon residue of the crude lipid was the lowest (1.6 wt. %) on the fifth day when the ratio of Antrodia camphora was 10%. When Chlorella vulgaris was co-cultured with 5% Antrodia camphorate on the fourth day, the particle size of Chlorella vulgaris is the lowest (5.55 μm). Moreover, the amount of crude lipid under co-cultivation of algae and fungus was higher than that under the condition of cultivating Antrodia camphora alone. Therefore, under the mechanism of synergistic symbiosis of algae-fungus, the fungus might enhance the growth of crude lipid of microalgae, probably because of abundant nutrients provided by their counterpart. It is inferred that symbiosis benefits of those two microorganisms were justified in this study.
Key words: Chlorella vulgaris, Antrodia camphorata, crude lipid, biodiesel, co-cultivation.
| Identifer | oai:union.ndltd.org:TW/107NTOU5484008 |
| Date | January 2018 |
| Creators | Hu, Xiao-Lan, 胡筱嵐 |
| Contributors | Cherng-Yuan Lin, 林成原 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 68 |
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