碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 107 / A suitable bone substitute is necessary in bone regenerative medicine. Hyaluronic acid (HA) has excellent biocompatible and biodegradable characteristics. Hence, HA is widely used in tissue engineering. Fucoidan (Fu) is a sulfated polysaccharide that contains L-fucose and sulfate, and can increase the activity of alkaline phosphatase (ALP) and even help in mineral deposition associated with osteoblast differentiation. In this study, HA and Fu were functionalized by grafting methacrylic groups in the chain backbone, respectively. The modified HA (Methacrylated HA, MHA) and Fu (Methacrylated Fu, MFu) were characterized by FTIR and 1H NMR spectroscopy to confirm the functionalization. The degree of methacrylation of MHA and MFu were 37.7% and 28.7%, respectively. Further, we evaluated the physicochemical properties of hydrogels formed from mixtures of photocrosslinkable methacrylated hyaluronan (MHA) and varying concentrations (0, 0.5 and 1%) of methacrylated fucoidan (MFu).
The initial hardness of MHA-MFu0.5 and MHA-MFu1 are 26±1.3 and 26±0.8 kPa, respectively, and there was no significant difference in statistical analysis. Moreover, the behaviour of MG63 osteoblastic-like cells was evaluated when cultured in contact with photo-crosslinked MHA-MFu hydrogel was investigated, being observed up to 28 days in culture. The results found that the cell viability was not significantly different on MHA-MFu0.5 and MHA-MFu1 after culture for 7 days. ALP activity and deposition of calcium were significantly higher on MHA-MFu1 than MHA. Hence, MHA-MFu composite hydrogel is a potential material for bone graft.
| Identifer | oai:union.ndltd.org:TW/107NTOU5613002 |
| Date | January 2019 |
| Creators | Chen, Jheng-Jie, 陳政杰 |
| Contributors | Huang, Pai-An, Hsu, Fu-Yin, 黃培安, 許富銀 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 48 |
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