碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 107 / Nowadays, applications of seaweed extractions have become a popular option on biological products. Therefore, extracting useful substances out of seaweeds and study its functions have been an important research project that scientists have paid attention on.
Fucoidan is a sulfated polysaccharide which is mainly extracted from brown algae. In this study, a simple and efficient way of hot water extraction was used to obtain the crude polysaccharides. This method is commonly used in the industrial. Further, agricultural waste was used to extract biogenic silica and it was synthesized into nanoparticles to be the carrier. In combination of the popular current drug delivery system (DDS), the carrier is doped with a dual imaging lanthanide metal, and loaded with fucoidan with better bioactivities such as anticancer activity, as the drug in the delivery system. However, the extracted fucoidan is expensive, taking advantage of the nanocarrier, we can reduce the amount of fucoidan usage and also the usage of expensive drugs.
The experiment is divided into three parts. The first part is to analyze the chemical properties and antioxidant of the extracted fucoidan. The second part, we endue the material with fluorescent and magnetic dual-imaging property by incorporating Eu3 + and Gd3 + during the synthesis of rMSN. The third part is to test the anti-cancer of rMSN-EuGd@Fucoidan. The drug delivery system rMSN-EuGd@Fucoidan which was synthesised in this research has shown cytotoxicy to A549 cancer cell. The result of cell viability test for fucoidan and rMSN-EuGd@Fucoidan is 58% and 47% respectively.IC50 value is 134.9 μg/mL and 57.33 μg/mL, concentration value 200 μg/mL is the summation of rMSN-EuGd and fucoidan. We can inverse calculate from the TGA data 54.5% to get the result of the fucoidan amount in rMSN-EuGd@Fucoidan, which is 109 μg. This result shows that we use much less amount of fucoidan. However, its cell toxicity against A549 cell is better than the fucoidan unloaded nanoparticles
| Identifer | oai:union.ndltd.org:TW/107NTOU5613018 |
| Date | January 2019 |
| Creators | Tsou, Min-Hsuan, 鄒旻軒 |
| Contributors | 林秀美 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 56 |
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