碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 107 / Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide, mostly diagnosed as locally advanced disease. HNSCC is a heterogeneous disease at clinical and molecular level, encompassing several tumors from hypopharynx, oropharynx, lip, oral cavity, nasopharynx, and larynx. High recurrence rate and regional metastases lead to major morbidity and mortality. HNSCC contains a small subpopulation of cells that exhibit a hallmark of CD44-expressing cancer stem cell (CSC) properties with self-renewal, multipotency, and a unique potential for tumor initiation. Therefore, the important knowledge obtained from CSC signaling and functional activation could provide new information regarding the design of novel drug targets to overcome current therapeutic drug resistance which will have significant treatment implications for head and neck cancer patients. Fucoidan, mainly derived from brown algae, is a complex of polysaccharides containing substantial percentages of L-fucose and sulfate ester groups. Fucoidan shows several remarkable biological activities that is involved in inhibiting proliferation and metastasis in breast cancer, colorectal cancer, prostate cancer, and lung cancer, demonstrating that the polysaccharide may increase the prognosis survival in cancers. This study aimed to evaluate the efficacy of fucoidan as a chemo-target agent to suppress the formation of CSC in HNSCC. Fucoidan was prepared from Sargassum hemiphyllum and characterized by HPLC and FTIR. Cytotoxic activity in fucoidan-treated SAS and Fadu cells was not observed, whereas viability of OECM1 cells were decreased after 48 h treatment of fucoidan. Cell migration and chemotaxis activity of HNSCC cells was suppressed after fucoidan treatment. Fadu cells were cultured under CSC formation condition, sphere is formed indicating the sternness property. The induction of Nanog, Sox2, Oct4 and CD44 was demonstrated in these spheres. However, the decrease of sphere was observed after fucoidan treatment in CSC formation. Moreover, the expression of Nanog and CD44 was inhibited after fucoidan treatment. Our findings demonstrate that fucoidan may reduce the formation of CSC to reduce tumorigenesis of HNSCC.
| Identifer | oai:union.ndltd.org:TW/107NTOU5613026 |
| Date | January 2019 |
| Creators | Ni, Chien-Yi, 倪千益 |
| Contributors | Tang, Shye-Jye, 唐世杰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 51 |
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