碩士 / 東吳大學 / 微生物學系 / 107 / Although the aquaculture industry's process of intensive production can increase the yield, it is also likely to cause a deterioration of the aquaculture environment. Few of the most common effects are an increased amount of the antibacterial agents and antibiotics used, raised incidence of drug-resistant bacteria and a decrease in public health security. To better maintain the sustainability of the aquaculture, the development and use of biological agents has received increasing attention. Previous study has shown that the Integrated Multi-Trophic Aquaculture System (IMTA) is potentially feasible for rearing Chanos chanos and the effectiveness of supplementation of photosynthetic bacteria Rhodovulum sulfidophilum as an aquaculture probiotic to this system was analyzed in this thesis. We measured the physicochemical properties of the pond waters and sediments of the IMTA, analyzed the community structure of bacteria and the presence of antibiotic resistance genes and enzymes involved in the nitrogen and sulfur cycling upon supplementation of the R. sulfidophilum. The results indicate that the application of photosynthetic bacteria can stabilize the flora, increase species diversity and richness, reduce the incidence of some drug resistance genes, and also change the presence of microorganisms involved in the elemental cycle. Based on the analysis of metagenome, we further learned that there are significant differences in the types and numbers of bacterial strains that carry these nitrogen-sulfur recirculating enzymes between the two types of sediment samples. It is expected that the provision of this information will bring us closer to the goal of optimizing the aquaculture environment.
Identifer | oai:union.ndltd.org:TW/107SCU00381003 |
Date | January 2019 |
Creators | CHANG, MAN-JUNG, 張曼容 |
Contributors | YING, JING-WEN, 應靜雯 |
Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
Language | zh-TW |
Detected Language | English |
Type | 學位論文 ; thesis |
Format | 80 |
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