碩士 / 國立臺北科技大學 / 化學工程與生物科技系化學工程碩士班 / 107 / With widespread abuse of antibiotics, bacterial resistance has increasingly become a serious threat. Phage is an alternative solution that can be used as a natural bacteriostatic agent. In this study, a phage solution resistant to Escherichia coli (DH5α) was isolated from seawater. The phage solution was uniformly mixed with alginate solution, and a fibrous alginate hydrogel scaffold with phage was prepared by three-dimensional printing. The phage activity assays, preservation efficiency, the bacteriostatic assays, tensile test, degradation test and cytotoxicity test were measured.
A phage solution resistant to E. coli (DH5α) was successfully isolated. The phage morphometric was observed by a transmission electron microscope (TEM), it was confirmed that the phage was a single morphometric after purification. The phage activity assays showed that the phage was successfully fixed on the scaffold and maintained good activity, and the preservation efficiency was about 87.66%. The bacteriostatic assays showed a very effective bacterial reduction, after immediate and 2 hours of contact (p<0.05). Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) were used to observe the bacteriostatic situation. In the incubation period of phage (20 minutes of infection time), showed didn’t bacterial reduction, but the phage was adsorbed on E. coli. During the phage lysis phase (120 minutes of infection time), showed a very effective bacterial reduction, and the E. coli membrane was broken. Cell DNA quantification showed that cells on the fibrous alginate hydrogel scaffold with phage increased over time, indicating that the phage-containing scaffold was not cytotoxic. The mechanical strength test confirmed that the phage was embedded in the sodium alginate scaffold without affecting the mechanical strength (P>0.05) and the degradation rate (P>0.05). In the future, it is expected that bacteria can be resistant to antibiotics when treating wound infections, and a solution is proposed.
| Identifer | oai:union.ndltd.org:TW/107TIT0006A085 |
| Date | January 2019 |
| Creators | SHEN, HAN-YU, 沈涵榆 |
| Contributors | LIN, HSIN-YI, 林忻怡 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 96 |
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