博士 / 臺北市立大學 / 運動科學研究所 / 107 / Background: Deep oceans, a possible site where lives are originated, may replenish the loss of molecular complexity associated with evolutionary sea-to-land migration. Here, we examined the effect of deep ocean mineral water (DOM) consumption in both lifelong effects in longevity, vitality, and tumor formation, and short-term effect in the inflammatory response of rat skeletal muscle. Methods: Study 1: Sprague Dawley rats (N = 194) were randomized into 4 groups: Control (C, N = 49), DOM (D, N = 48), Fructose (F, N = 49), and Fructose + DOM (FD, N = 48) consuming DOM with or without 11% fructose since 6 months of age until naturally death. Study 2: Sprague Dawley rats (N = 47) were randomized into 4 groups: Control (C, N = 12), Fructose (F, N = 12), Fructose + Exercise (FE, N = 12), and Fructose + Exercise + DOM (FED, N = 11). Soleus muscles were analyzed 24 h after an acute bout of downhill running following 9 weeks of treatments. Fructose 11% was used in both studies since it is a common concentration in beverages. Results: Study 1: DOM and fructose drink had no effect on longevity. However, DOM significantly lowered tumor incidence (necropsy) by 39 %, along with lowered body weight since 19 months of age and increased locomotive activity observed at 18 months of age compared with the C group (C vs. D, P < 0.05). Reduced and oxidized glutathione (GSH/GSSG) ratio did not differ between groups but decreased with age, followed by increased above the 6 months level at 24 months of age. This increase in GSH/GSSG is associated with significantly lower tumor incidence. Study 2: Leukocyte infiltration and TNF-α mRNA of muscle in the FE group were 5 times and 4 times above the F group, respectively (P < 0.05). Both markers were significantly lower in the FED group compared with the FE group (P < 0.05). Interleukin-10 (IL-10) mRNA of muscle in the fructose-fed groups was 9 times higher than that in the C group (P < 0.05). The GSH/GSSG ratio of muscle in the F group was 40% lower than that in the C group (P < 0.05). However, this ratio was similar to the control in the presence of DOM. Conclusion: Consuming DOM and fructose (11%) can neither effect lifespan nor toxic in rats. Decreased tumor incidence by DOM implicates a new role in cancer prevention. Moreover, the results of short-term study implicate prolonged fructose drinking modulates inflammatory balance and the application of DOM can minimize exercise-induced inflammatory response in skeletal muscle.
| Identifer | oai:union.ndltd.org:TW/107UT005421001 |
| Date | January 2019 |
| Creators | Suchada Saovieng, Suchada Saovieng |
| Contributors | Kuo, Chia-Hua, 郭家驊 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 96 |
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