The ribonucleotides in the chorioallantoic membrane of 12-day-old chick embryos have been isolated by ion-exchange chromatography and characterized by their spectrophotometric and paper chromatographic properties. The following nucleotides were identified: adenosine-5' phosphate (AMP), uridine-5' phosphate (UMP), cytidine-5' phosphate (CMP), uridine-5' diphosphate galactose (UDPGal), uridine-5' diphosphate N-acetyl hexosamine (UDPNAHexosamine), guanosine-5' phosphate (GMP), cytidine-5' diphosphate (CDP), uridine-5' diphosphate (UDP), adenosine-51 diphosphate (ADP), guanosine-5' diphosphate (GDP), cytidine-5' triphosphate (CTP), uridine-5' triphosphate (DTP), adenosine-5' triphosphate (ATP), and guanosine-5' triphosphate (GTP).
Quantitative determinations of these nucleotides were made on the basis of their ultraviolet absorption at 260 mμ. Similarly, concentrations of these nucleotides were estimated in 12-day-old chorioallantoic membranes after infection with vaccinia virus.
Larger amounts of ribonucleoside-5' phosphates were present in the infected tissue at 4 and 12 hours after infection. The amounts of ribonucleoside-5' triphosphates were decreased. In tissues where, it is believed, synchronous infection occurred, the amounts of ribonucleoside-5' diphosphates and triphosphates were markedly lower than in controls after 12 hours of infection.
Infection in the presence of tritium₌labelled thymidine showed that the amount of labelled thymidine-5' mono-, di-, and triphosphates had increased after 4 hours and that the amounts of these nucleotides subsequently decreased. / Science, Faculty of / Microbiology and Immunology, Department of / Graduate
| Identifer | oai:union.ndltd.org:UBC/oai:circle.library.ubc.ca:2429/39702 |
| Date | January 1964 |
| Creators | Wylie, Vivian |
| Publisher | University of British Columbia |
| Source Sets | University of British Columbia |
| Language | English |
| Detected Language | English |
| Type | Text, Thesis/Dissertation |
| Rights | For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use. |
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