Prenatal alcohol exposure is known to have many profound detrimental effects on human fetal development (fetal alcohol spectrum disorders), which may manifest into lifelong disabilities. Although hearing deficiency is a recognized effect, how alcohol affects the auditory/vestibular systems has not been well studied. This is the first study that used the zebrafish, Danio rerio, as a model organism to investigate morphological effects of alcohol on the developing octavolateral system (auditory, vestibular and lateral line). Zebrafish embryos of two hours post fertilization (hpf) were treated in 2% alcohol for 48 hours and screened at 72 hpf for morphological defects of the inner ear and lateral line neuromasts. Inner ear size and otoliths of zebrafish from both alcohol-treated and control groups were examined using light microscopy. Zebrafish were stained with fluorescent vital dyes to visualize lateral line hair cells using confocal microscopy. The size of neuromasts and length of kinocilia were measured using scanning electron microscopy. Results reveal that alcohol treatment during the early development impairs the formation of otoliths, neuromasts and their hair cells, as well as ear size and kinocilium length. We observed several otolith phenotypes including zero, one, two abnormal, two normal and multiple otoliths for alcohol-treated zebrafish. Fetal alcohol exposure appears to severely impact the size of both anterior and posterior lateral line neuromasts. Our results indicate that early fetal alcohol exposure most likely results in functional defects of the octavolateral system due to inner ear and lateral line dysmorphology.
| Identifer | oai:union.ndltd.org:UMIAMI/oai:scholarlyrepository.miami.edu:oa_theses-1280 |
| Date | 07 August 2011 |
| Creators | Zamora, Lilliann Y |
| Publisher | Scholarly Repository |
| Source Sets | University of Miami |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Open Access Theses |
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