Orientadora: Maria Célia Bertolini / Banca: Nilce Maria Martinez Rossi / Banca: Rafael Silva Rocha / Banca: Carlos Takeshi Hotta / Banca: Iran Malavazi / Resumo: The fungus Neurospora crassa, a model organism in studies of gene expression, metabolism, photobiology and circadian rhythm, is able to respond and adapt to different environmental stresses, such as heat shock, pH changes, nutrient limitation, osmotic stress, and others. Besides that, N. crassa has the genome sequenced and collections of knocked-out strains are avalaible to the scientific community. A systematic screening analysis performed with mutant strains in genes encoding transcription factors led to identify proteins involved in the glycogen metabolism regulation in this fungus. Glycogen and trehalose are storage carbohydrates that functions as a carbon and energy reserve. Trehalose can also protect membranes and proteins, increasing the tolerance to adverse conditions. In this work, some transcription factors were functionally characterized regarding their role in glycogen and trehalose metabolism regulation. The first condition investigated was the influence of the circadian clock in the glycogen metabolism. We observed that the glycogen accumulation and the expression of genes encoding glycogen synthase (gsn) and glycogen phosphorylase (gpn) are rhythmic in a wild-type strain and dependent on the FREQUENCY (FRQ) oscillator, the core component of the N. crassa circadian clock. The VOS-1 transcription factor, that is controlled by clock and can act in the connection between clock and glycogen metabolism, binds to gsn and gpn promoters rhythmically. However, the expres... (Resumo completo, clicar acesso eletrônico abaixo) / Doutor
| Identifer | oai:union.ndltd.org:UNESP/oai:www.athena.biblioteca.unesp.br:UEP01-000873444 |
| Date | January 2016 |
| Creators | Virgilio, Stela. |
| Contributors | Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Química. |
| Publisher | Araraquara, |
| Source Sets | Universidade Estadual Paulista |
| Language | Portuguese |
| Detected Language | English |
| Type | text |
| Format | 221 f. |
| Relation | Sistema requerido: Adobe Acrobat Reader |
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