Orientador: Edilson Ervolino / Coorientadora: Roberta Okamoto / Banca: Leonardo Perez Faverani / Banca: Rogerio Leone Buchaim / Resumo: OBJETIVOS: Os objetivos do presente estudo foram avaliar o efeito da terapia com laser em baixa intensidade (LLLT) e da terapia fotodinâmica antimicrobiana (aPDT) no processo de reparo alveolar de ratas com os principais fatores de risco para a osteonecrose dos maxilares associada à terapia medicamentosa (ONM-M). MATERIAIS E MÉTODOS: Ratas senis (n=29) foram distribuídas nos grupos: SAL, ZOL, ZOL/LLLT e ZOL/aPDT. Durante sete semanas, a cada dois dias, administrou-se pela via intraperitoneal 0,45ml de NaCl 0,9% (SAL) ou 0,45ml desta mesma solução acrescida de 100μg/Kg de zoledronato (ZOL, ZOL/LLLT e ZOL/aPDT). Decorridas três semanas, realizou-se a exodontia do primeiro molar inferior esquerdo. Nos grupos ZOL/LLLT e ZOL/aPDT as ratas foram submetidas respectivamente a LLLT ou aPDT aos 0, 2 e 4 dias pós exodontia. A eutanásia foi realizada 28 dias após a exodontia. No sítio de extração dental foram realizadas análises: histopatológica do processo de reparação tecidual, histométrica da área de tecido ósseo neoformado (ATO) e imunoistoquímica direcionada para os seguintes biomarcadores: PCNA, BAX, C3C, TNFα, IL-1β, IL-6, HIF-1α, VEGF, CD31, BMP2/4, RUNX-2, OCN, OPG, RANKL e TRAP. RESULTADOS: Os grupos tratados com zoledronato apresentaram maior imunomarcação para OPG e menor imunomarcação para RANKL e TRAP. Em ZOL observou-se áreas de osteonecrose, comprometimento da reparação tecidual, menor ATO, menor imunomarcação para PCNA, HIF-1α, VEGF, CD31, BMP2/4 e OCN, e maior ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: OBJECTIVES: The aim of this study was to evaluate the effects of Low Level Laser Therapy (LLLT) and antimicrobial photodynamic therapy (aPDT) in the a lveolar bone repair in rats with the high risk factors for Medication - Related Osteonecrosis of the Jaw (MRONJ). MATERIALS AND METHODS: Senile rats (n = 29 ) were distributed into four grou ps: SAL, ZOL, ZOL/LLLT and ZOL/aPDT. For seven weeks, every two days, 0,45ml 0.9% NaCl (SAL) or 0,45ml of this same solution plus 100μg/kg zoledronate (ZOL, ZOL/LLLT and ZOL/aPDT) were administered intraperitoneally. After three weeks, the first lower left molar was extracted. In ZOL/LLLT and ZOL/aPDT groups, the rats were submitted to LLLT or aPDT therapy at 0, 2 and 4 days after tooth extraction. Euthanasia was performed 28 days after tooth extraction. In dental extraction site was performed the histopath ology analysis of the tissue repair, histometric analysis of newly formed bone area (NFB) and immunohistochemistry related to the following biomarkers: PCNA, BAX, C3C, TNFα, IL - 1β, IL - 6, HIF - 1α, VEGF, CD31, BMP2/4, RUNX - 2, OCN, OPG, RANKL and TRAP. RESULT S: The groups treated with zoledronate showed higher immunolabeling for OPG and lower immunolabeling for RANKL and TRAP. In ZOL group was observed areas of osteonecrosis, impairment of wound healing, lower ATO, lower immunolabeling for PCNA, HIF - 1α, VEGF, CD31, BMP2/4 and OCN, and higher immunolabeling for BAX, C3C, TNFα, IL - 1β, IL - 6 and RUNX - 2 when compared to the SAL group. ZOL/LLLT showed improvement in some parameters regarding to ZOL (ATO, PCNA, TNFα, HIF - 1α, VEGF, CD31 and RUNX - 2), however, few parame ters were similar to the SAL (PCNA, HIF - 1α, VEGF, CD31 and RUNX - 2). ZOL/aPDT did not present areas of osteonecrosis and the tissue repair did not differ significantly from the SAL groups, as well as the following ...(Complete abstract electronic access below) / Mestre
| Identifer | oai:union.ndltd.org:UNESP/oai:www.athena.biblioteca.unesp.br:UEP01-000874506 |
| Date | January 2016 |
| Creators | Statkievicz, Cristian. |
| Contributors | Universidade Estadual Paulista (Unesp) Faculdade de Odontologia |
| Publisher | Araçatuba, |
| Source Sets | Universidade Estadual Paulista |
| Language | Portuguese |
| Detected Language | English |
| Type | text |
| Format | 96 f. : |
| Relation | Sistema requerido: Adobe Acrobat Reader |
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