This project revolves around the measurement of T1 and T2 relaxation times in the ratbrain, in a multi-center way. That is to say, elaborate an efficient protocol to calculate highresolution 3D map of the brain. This protocol should be applied in different centers andreturn similar results. Finally, procedures should be defined to ease the collaborationbetween the different centers. The first step consisted in in vitro experiments, in whichdifferent sequences were tested. It resulted that the MDEFT sequence with inversionpreparation (MPRAGE) gives the best results in the shortest time for T1. For T2, theMSME sequence was chosen. The next step moved on in vivo experiments on three rats inorder to get used to manipulating living animals and make new adjustments. As thephysiology is not the same on in vitro and in vivo experiments, some parameters had to beslightly adapted. Once the final 2h-protocol was established, it was tested on a populationof ten rats. Experiments were made at the GIN and CRMBM. Different fitting pipelineswere tried (GIN, CRMBM, MIRCEN). The brain was segmented into different regions. Itresulted that the GIN and CRMBM pipelines return the same T1 values using the differentdatasets. The MIRCEN pipeline under-estimates by 200 ms. The three pipelines return similar T2 values. The GIN and CRMBM datasets provide comparable T1 values, but theGIN center presents slightly higher T2 values. Regarding the multi-center collaboration,the different pipelines were ported to the VIP platform so that the scientific community caneasily reuse them.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:kth-232040 |
| Date | January 2018 |
| Creators | DERUELLE, Tristan |
| Publisher | KTH, Skolan för kemi, bioteknologi och hälsa (CBH) |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | TRITA-CBH-GRU ; 2018:73 |
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