Galvanotaxis is the movement of cells in an applied electric field. The first steps to design a chip for observations of galvanotaxic behavior of cells were done in this work. The chip is a miniaturised system of previous larger galvanotaxis systems and uses materials which are thought to be biocompatible. The system was constructed on microscope slides with a channel in PDMS with adjacent polymer electrodes. The polymer electrodes were made from poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS), glycerol and Silquest A-187. The PEDOT:PSS electrodes were connected with either an evaporated metal electrode of titanium and gold or a gold net. Systems with PEDOT:PSS are neutralised when put in excessive amount of PBS (pH=7.4) for 24 hours. The final system had a channel with dimension length=14 mm, width=0.5 mm and height=0.25 mm. PEDOT:PSS worked as an electrode material and the achieved electric field through the channel was between 55 V/m and 160 V/m with an applied voltage of 1 V. The decrease of the electric field within the first hour was between 10 % and 30%. Further development of this system could give an easy way to observe galvanotaxic behaviour of cells or an instrument that can distinguish between different cell types.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:liu-70081 |
| Date | January 2011 |
| Creators | Bengtsson, Katarina |
| Publisher | Linköpings universitet, Institutionen för fysik, kemi och biologi, Linköpings universitet, Tekniska högskolan |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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