Background: More than 700 oral bacterial species have been found and together they make out the oral microbiota. Specific species have shown to correlate with various oral diseases such as caries and periodontitis, but also systemic diseases. Most studies have looked at the whole microbiota but the knowledge about tooth site-specific variation within supragingival plaque after lack of oral hygiene in healthy participants is limited. Aim: This pilot study aimed to characterize variations in the supragingival plaque with regards to the; anterior (incisors and canines) and posterior (molars and premolars) teeth, upper and lower jaw, and left versus right tooth arches. Method: After three days of accumulating plaque, supragingival tooth biofilm was collected from 16 different tooth sites, from six healthy participants. Bacterial DNA was extracted, and 16s rRNA gene (V3-V4) was amplified by PCR and sequenced on an Illumina MiSeq platform. Sequences were blasted and taxonomically allocated using the Human Oral Microbiome Database. Result: In summary, 50 species showed a difference between the anterior and the posterior region, 30 species differed between the upper and lower jaw, and three species differed between the left and right sides. Conclusion: This study indicates a difference in oral microbiota composition in supragingival plaque on different tooth regions. These findings emphasize the choice of method when analyzing the oral microbiota—also highlighting the importance of further understanding the dynamic forces driving local enrichment and reduction of specific species.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:umu-201263 |
| Date | January 2022 |
| Creators | Albertsson, Hanna, Isik, Melina |
| Publisher | Umeå universitet, Institutionen för odontologi |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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