Eimeria tenella is a eukaryotic, intracellular parasite that, along with six other Eimeria species, causes coccidiosis in chickens. This disease can result in weight loss or even death and is estimated to cause 2 billion euros of damages to the chicken industry each year. While much is known of the life cycle of E. tenella in the chicken, less is known about molecular mechanisms of infection and the chicken immune response. In this study, we produced a pipeline for dual RNA-sequencing analysis of a mixed chicken and E. tenella dataset. We then carried out an analysis on an in vitro infection of the chicken macrophage HD-11 cell line. This was followed by a differential expression analysis across six time points, 2, 4, 12, 24, 48, and 72 hours post-infection, in order to elucidate these mechanisms. The results showed clear patterns of expression for the chicken immune genes, with strong down-regulation of genes across the immune system at 24 hours and a repetition of early patterns at 72 hours, indicating that reinfection by a second generation of parasite cells was occurring. Several genes that may have important roles in the immune reaction of the chicken were identified, such as MRC2, ITGB3 and ITGA9, along with genes with known roles, such as TLR15. The expression of surface antigen genes in E. tenella was also examined, showing a clear upregulation in the late stages of merogony, suggesting important roles for merozoites. Finally, a co-expression analysis was carried out, showing considerable co-expression among the two organisms. One of the gene co-expression networks identified appeared to be enriched with both infection specific genes from E. tenella and chicken immune genes. These results, along with the pipeline, will be used in further studies on E. tenella infections and bring us closer to the eventual goal of a vaccine for coccidiosis.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-413432 |
| Date | January 2020 |
| Creators | Sigurðarson Sandholt, Arnar Kári |
| Publisher | Uppsala universitet, Institutionen för biologisk grundutbildning |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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