Levels of Immunoglobulin G (IgG)-subclasses are analyzed when patients have reoccurring infections and in order to follow the treatment of IgG4 related disease. The measured quantity of IgG-total can be within the reference interval even if the patient has a deficiency in one of the IgG-subclasses. Due to this Sundsvall’s hospital plans to begin analyzing IgG-subclasses. The aim was to verify the performance level of the analysis IgG-subclasses (IgG1-4) with Siemens Atellica NEPH 630. The method was verified by evaluating the method’s precision and by comparing the sum of IgG-subclasses with the quantity of IgG-total analyzed on Cobas c502. In addition, the reference intervals provided by Siemens were evaluated using samples from blood donors. The study evaluated whether there was a correlation between infection with SARS-CoV2 and a deficiency in IgG-subclasses. The verification began by performing quality control twice daily over a period of four weeks. The IgG-subclasses test was performed on blood donor samples with Siemens Atellica NEPH 630 for evaluation of the reference values. The coefficient of variation was less than 6 % for all four subclasses. The reference values for IgG1, IgG2, IgG3, and IgG4 are all in alliance with the reference values provided by Siemens. The sum of IgG-subclasses corresponded well with IgG-total with a correlation value (R) 0.82. No correlation was found between IgG deficiency and infection with SARS-CoV2. The validation of the analysis of IgG-subclasses was successful with quality measurements within the supplier’s intervals. No adjustments will be needed for the reference intervals.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-445446 |
| Date | January 2021 |
| Creators | Sayed, Rama |
| Publisher | Uppsala universitet, Institutionen för medicinsk cellbiologi |
| Source Sets | DiVA Archive at Upsalla University |
| Language | Swedish |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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