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Detection of celery (Apium graveolens) in food with Real-Time PCR

Directive EC 2003/89/EC of the European Parliament and of the Council states that certain ingredients and products derived there of known to cause allergen reactions must always be declared. Furthermore labelling is mandatory irrespective of the amount included. The National Food Administration therefore needs methods for monitoring the presence of allergens in food. Methods already exist for most of the allergens on the EU-list, but an operational method for celery (Apium graveolens) is missing. A specific DNA-method was developed, based on TaqMan Real-Time PCR with the celery mannitol dehydrogenase gene as target sequence. The analysis was started with homogenisation of the sample followed by extraction of DNA. The Real-Time PCR method was shown to be specific for celery, producing a 113 bp fragment with two celery varieties and negative results with other closely selected species commonly present together with celery in food products (12 samples). The detection limit was 2-20 pg DNA, which corresponds to 1-7 haploid genome copies. When evaluated with model samples of celery in meat, a detection limit of less than 0,01 % was determined. When used to analyse food products from the market, six out of seven products declared to contain celery were correctly identified as positive.

Identiferoai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-7130
Date January 2006
CreatorsAfshari Kashanian, Elisa
PublisherUppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, Uppsala : Universitetsbiblioteket
Source SetsDiVA Archive at Upsalla University
LanguageEnglish
Detected LanguageEnglish
TypeStudent thesis, info:eu-repo/semantics/bachelorThesis, text
Formatapplication/pdf
Rightsinfo:eu-repo/semantics/openAccess

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