The Biochemical Library (BCL) is a protein structure prediction algorithm developed in the Meiler Lab at Vanderbilt University based on the placement of secondary structure elements (SSEs). This algorithm incorporates sparse experimental data constraints from nuclear magnetic resonance (NMR), Cryo- electron microscopy (CryoEM), and electron paramagnetic resonance (EPR), to restrict the conformational sampling space but does not have the capability to use Small Angle X-Ray / Neutron data. This dissertation delineates my work to add this capability to BCL::Fold. Specifically I show and show for what type of structures SAXS/SANS experimental data improves the accuracy BCL:: Fold and importantly where it does not. Furthermore, in collaboration with Oak Ridge National Labs, I present my work on structural determination of the Cellulose Synthase Complex in Arabidopsis Thaliana.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-03282016-121917 |
| Date | 28 March 2016 |
| Creators | Putnam, Daniel Kent |
| Contributors | Jens Meiler, Loukas Petridis, Douglas P. Hardin, Martin Egli, Thomas A. Lasko |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-03282016-121917/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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