New technologies for model biological systems aid in drug development and toxicology by providing an in vitro environment that closely mimics in vivo conditions but require new instrumentation to meet needs in monitoring and analysis. Techniques adapted from the existing Multianalyte Microphysiometer, an instrument used for the quantitative analysis of cell metabolism, were applied in the optimization of a screen-printed platinum electrode platform capable of monitoring levels of glucose, lactate, oxygen, and solution acidification in complex mixtures such as cell medium for integration into biological model systems. Various modifications including enzyme and metal films provide sensitivity and selectivity to the electrodes. Improvements were made in the function of the screen-printed electrode platform and preliminary steps in the integration of the electrodes with a hollow-fiber bioreactor intended to simulate the environment of the blood-brain barrier were performed. Challenges in the interfacing of biological model systems with fundamentally unsterile instruments are identified and addressed.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-03282016-235557 |
| Date | 11 April 2016 |
| Creators | Cognata, Andrew David |
| Contributors | Dr. David Cliffel, Dr. John McLean |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-03282016-235557/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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