The effects of DNA damage signaling on the DNA replication of the polyomavirus SV40 are examined in this dissertation. Infection of monkey cells with SV40 results in large amounts of cellular DNA damage signaling through the cellular kinases ataxia telangiectasia-mutated (ATM) and ATM and Rad3-related (ATR). Literature pertaining to DNA replication, DNA repair, DNA damage signaling, and polyomaviral replication is first discussed. This is followed by a number of experiments testing the molecular mechanisms that necessitate DNA damage signaling by ATM and ATR during SV40 infection. Furthermore, the contribution of ATM and ATR to SV40-induced cell cycle arrest and repair protein recruitment to viral DNA replication centers is explored. After an examination of how my experiments fit in with relevant manuscripts, I discuss the possible consequences of the aberrant products found upon inhibition of DNA damage signaling during SV40 DNA replication and how my current studies can be advanced. Additionally, the connection between DNA damage signaling and DNA repair and the implications of my dissertation to cellular DNA replication are discussed.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-08232013-173001 |
| Date | 06 September 2013 |
| Creators | Sowd, Gregory Alan |
| Contributors | Ryoma Ohi, David Cortez, Terence Dermody, Ellen Fanning, Katherine Friedman |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-08232013-173001/ |
| Rights | restricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
Page generated in 0.0023 seconds