Bovine morulae (day 6: n=257) were obtained to evaluate the [effect of immunoglobulins (Ig) on early bovine embryo development in vitro. Fifty-four cows superovulations were conducted in 36 cows with follicle stimulating hormone. Embryos were collected by non-surgical procedures and morphologically evaluated and randomly assigned to culture. Embryos were cultured in Ham's F-10 containing 10% (6.4 mg/ml) steer serum (SS), 1% (.64 mg/ml) bovine gamma . globulins (GG), 1% (.64 mg/ml) bovine IgG, 1% (.64 mg/ml) bovine 1gM, 10% SS plus 1% GG, 10% SS plus 1% 1gG, or 10% SS plus 1% 1gM. Embryos were cultured to the hatched blastocyst stage or degeneration and evaluated at 12 h intervals. / Master of Science
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/41575 |
| Date | 12 March 2013 |
| Creators | Sedano, Rodolfo Canseco |
| Contributors | Dairy Science, Gwazdauskas, Francis C., Vinson, William E., Barnes, Michael A., White, John M. |
| Publisher | Virginia Tech |
| Source Sets | Virginia Tech Theses and Dissertation |
| Detected Language | English |
| Type | Thesis, Text |
| Format | vii, 100 leaves, BTD, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
| Relation | OCLC# 13435211, LD5655.V855_1985.S432.pdf |
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