Purpose: To determine the association of plasma and aqueous humor selenium with glaucoma; and to determine those factors influencing biological levels of selenium in patients with glaucoma and cataractMethods: 47 primary open angle glaucoma (POAG) cases and 54 controls were recruited from surgery patients at the University Physician's Ophthalmology Clinic in Tucson, Arizona. Aqueous humor and plasma selenium concentration was determined by high performance liquid chromatography ion channel plasma-mass spectrometry (HPLC ICP-MS). Potential confounders were assessed via questionnaire. Outcome measures included the odds of glaucoma in relation to plasma selenium and aqueous humor selenium. Factors driving plasma and aqueous humor selenium in the study population were determined via linear regression.Results: After adjustment for risk factors and multiple outcomes, the odds of glaucoma in the highest tertile of plasma selenium (OR = 13.51; p=.03) and the middle tertile of aqueous humor selenium (OR = 0.05; p=0.02) were significantly associated with glaucoma. Selenium concentration in plasma and aqueous humor was primarily driven by metabolic factors (cancer, DMII, and ARMD).Conclusions: Although a causal pathway cannot be inferred from the analysis, it may be prudent to explore these relations in a larger sample in varying areas of geographic selenium distribution. Such information could be helpful in examining a larger study population and comparing biological data. Evaluating a subject's selenium levels over time and in relation to glaucoma onset could also lend pertinent informationDriving forces behind selenium concentrations in this population are mainly metabolic in nature. Selenium levels fluctuate in most tissue as it is metabolized by the body. Selenium supplementation is a significant predictor but this effect is small and may be transient as supplementation only temporarily increases selenium pools. It is interesting to note that sex is a significant predictor of aqueous humor selenium but not of plasma selenium. In this model, female sex predicts a decrease in the selenium within the aqueous humor. This point should be explored in future studies that are powered to discern possibly subtle differences that sex plays in relation to selenium concentrations in plasma and aqueous humor and its possible role in glaucoma.
| Identifer | oai:union.ndltd.org:arizona.edu/oai:arizona.openrepository.com:10150/195335 |
| Date | January 2008 |
| Creators | Bruhn, Roberta L |
| Contributors | Chen, Zhao, Ranger-Moore, James, Stamer, W. Daniel, Gandolfi, A. Jay |
| Publisher | The University of Arizona. |
| Source Sets | University of Arizona |
| Language | English |
| Detected Language | English |
| Type | text, Electronic Dissertation |
| Rights | Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. |
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