Neutrophils have been implicated in exacerbating ischemia/reperfusion injury in hearts. This study was undertaken to compare the adhesion of isolated rabbit neutrophils to rabbit coronary endothelial cells grown in culture under static and flow conditions. Also, the effects of a platelet activating factor (PAF) antagonist (SDZ 64-412) were evaluated in both systems along with monoclonal antibodies (mab) against specific adhesion proteins in the static system. Comparable concentration response curves for SDZ 64-412 were obtained in both systems with a greater decrease in adhesion seen in the flow system versus the static system. Both systems showed a significant inhibition of adhesion with increased concentration. The mabs against CD11a, CD11b, CD18, and ICAM reduced adhesion to 94.8%, 76.5%, 72.6%, and 26.7%, respectively. In conclusion, neutrophil/coronary endothelial cell adhesion can be quantified in both static and flow systems. Also, SDZ 64-412 effectively inhibits the adhesion of these two cell types to an even greater extent than certain adhesion protein mabs.
| Identifer | oai:union.ndltd.org:arizona.edu/oai:arizona.openrepository.com:10150/278179 |
| Date | January 1992 |
| Creators | Stringer, Steven Kennith, 1955- |
| Contributors | Larson, Douglas F. |
| Publisher | The University of Arizona. |
| Source Sets | University of Arizona |
| Language | en_US |
| Detected Language | English |
| Type | text, Thesis-Reproduction (electronic) |
| Rights | Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. |
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