Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host-microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.
| Identifer | oai:union.ndltd.org:arizona.edu/oai:arizona.openrepository.com:10150/614760 |
| Date | 11 May 2016 |
| Creators | Shah, Pranjul, Fritz, Joëlle V., Glaab, Enrico, Desai, Mahesh S., Greenhalgh, Kacy, Frachet, Audrey, Niegowska, Magdalena, Estes, Matthew, Jäger, Christian, Seguin-Devaux, Carole, Zenhausern, Frederic, Wilmes, Paul |
| Contributors | Univ Arizona, Ctr Appl Nanobiosci & Med, Univ Arizona, Dept Basic Med Sci |
| Publisher | NATURE PUBLISHING GROUP |
| Source Sets | University of Arizona |
| Language | English |
| Detected Language | English |
| Type | Article |
| Rights | This work is licensed under a Creative Commons Attribution 4.0 International License. |
| Relation | http://www.nature.com/doifinder/10.1038/ncomms11535 |
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