Three major topics will be discussed in this dissertation. The first is an optical biosensor for specific diagnosis of bacterial skin and wound infection, followed by a paper microfluidic assay and accompanying monitoring device for monitoring blood coagulation and determining patient-specific heparin and protamine dosing. The final work to be discussed is ongoing work involving the detection of circulating tumor cells (CTCs) using a paper microfluidic detection platform.
All of these works involve the development of biosensors for the simultaneous advancement and simplification of diagnosis and analysis of blood and bacterial infection. The aims of each of these projects included significantly decreasing the time to diagnosis and decreasing the reagents, laboratory space, personnel, and other resources needed for detection and diagnosis.
The first works are focused on the design, development, and testing of an optical biosensor for the immediate detection of bacterial skin and wound infection, including diagnosing the specific species of bacteria responsible for the infection. The optical biosensor developed allows for diagnosis of a bacterial infection on skin or in a wound in as little as three seconds, in a contact-free, reagent-free manner.
The second work focused on the design, development, and testing of a paper microfluidic assay and accompanying Raspberry Pi-based monitoring device for use before, during, and after surgeries requiring the use of cardiopulmonary bypass. The assay monitors the extent of blood coagulation of a whole blood sample and determines patient-specific dose response curves of an anticoagulant and its reversal agent.
The final work discussed focuses on developing a paper microfluidic assay for the detection of CTCs from whole blood samples. The goal of this work is to detect multiple morphologies of CTCs from whole blood samples to provide insight on patient prognosis in a rapid, low resource manner.
| Identifer | oai:union.ndltd.org:arizona.edu/oai:arizona.openrepository.com:10150/626360 |
| Date | January 2017 |
| Creators | Sweeney, Robin Emily, Sweeney, Robin Emily |
| Contributors | Yoon, Jeong-Yeol, Yoon, Jeong-Yeol, Uhlmann, Donald, Wong, Raymond, Viswanathan, Virinchipuram |
| Publisher | The University of Arizona. |
| Source Sets | University of Arizona |
| Language | en_US |
| Detected Language | English |
| Type | text, Electronic Dissertation |
| Rights | Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. |
Page generated in 0.0023 seconds