Tumor biologist have long appreciated that both cell to
cell and cell to extracellular matrix (ECM) interactions are
involved in the invasive and metastatic events that are
characteristic of malignancy. Cancer cell attachment to and
invasion of an ECM has been associated with metastatic
potential of cell lines of the Dunning rat prostate model.
It was postulated that differences observed in the
metastatic potential of four Dunning cell lines may
correlate with cell-matrix interactions. Four cell lines,
highly metastatic ML, MLL, AT-3 and non-metastatic AT-1 were
studied. The adhesive, invasive and chemoinvasive
capability of each cell line was compared. Cell adhesion
was examined by plating the cells on plastic dishes coated
with various components of the ECM (fibronectin, laminin and
collagen) as well as EHS Natrix (a natural ECM) . Invasion
was determined by examining cells ability to traverse a
matrigel barrier. Correlations were found between the
cells' adhesive and invasive abilities in response to the
ECM. These observations suggest that ECM components are
highly involved in prostate cancer cell activities and loss
may contribute to tumor progression and metastasis.
| Identifer | oai:union.ndltd.org:auctr.edu/oai:digitalcommons.auctr.edu:dissertations-4844 |
| Date | 01 March 1995 |
| Creators | Donald, Carlton Dewitt |
| Publisher | DigitalCommons@Robert W. Woodruff Library, Atlanta University Center |
| Source Sets | Atlanta University Center |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | ETD Collection for AUC Robert W. Woodruff Library |
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