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Following the fate of proteinaceous material in soil using a compound-specific 13C-and 15N-labelled tracer approach

Organic nitrogen containing compounds represent the vast majority of natural N inputs to soils and of these, proteins, peptides and amino acids are the most significant. In order to facilitate the elucidation of both the nature and rates of the processes by which these vital nutrients are transformed in soils, an understanding of their fate at a mole; level is vital. This thesis aimed to utilise soil incubation experiments with dual (¹⁵N- and ¹³C-) labelled substrates, i.e. a suite of amino acids, individual amino acids and more complex substrates, i.e. protein and cow dung, as isotopically labelled trace enabling their N and C to be followed over time. A molecular level approach was adopted which included the application of chemically and isotopically defined substrates to soil and the use of compound-specific stable isotope (N and C) analysis to trace the fate of label into the soil amino acids (and in selected cases phospholipids, C only).

Identiferoai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:503943
Date January 2009
CreatorsKnowles, Timothy David James
PublisherUniversity of Bristol
Source SetsEthos UK
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation

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