AIMS: The aim of the current project was to determine if lycopene could modulate T-cell function and activity. METHODS: 1) Peripheral blood mononuclear cells (PBMC) were isolated from healthy adults and incubated with lycopene-enriched liposomes (0.0-1.2 μg/ml) then activated with or without the mitogens Con A, anti-CD3 and LPS and cultured for various lengths of time before measuring CD3, CD4, CD8, CD69, CD11a and CD25 expression. the production of IL-1β, IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ, TGF-β and TNF-α were also measured along with the percentage of FoxP3 and IL-10 positive T-regulatory cells. 2) <i>Ex-vivo</i> expression of CD11a, CD49d, CD54, CD3 and CD69, as well as Con A stimulated proliferation were measured in subjects before and after a 12 week tomato-food and lycopene intervention study. RESULTS: Lycopene reduced PBMC proliferation and CD69 expression irrespective of the type of T-cell considered. Lycopene reduced IL-2, IL-10, IL-17 and IFN-γ but increased IL-1β and TNF-α production. lycopene also reduced the number of CD4<sup>+</sup>/CD25<sup>+</sup> cells present as well as those positive for IL-10. Other parameters were not affected. CONCLUSION: Lycopene reduces T-cell activity by mechanisms dependent on early cell activation but this modulation is not specific to T-cell types. the data support a potential beneficial effect of lycopene in the reduction of atherosclerosis through the modulation of T-cell function.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:521339 |
| Date | January 2010 |
| Creators | Mills, Lynsey M. |
| Publisher | University of Aberdeen |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=130840 |
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