Colostrum is the first milk produced after birth, is particularly rich in antibodies, antibacterial proteins and bioactive substances. Recent studies have suggested that colostrum might be useful in treating gut damage. The value of colostral preparations in patients taking long-term non -steroidal anti- inflammatory drugs (NSAIDs) was examined by studying the influence of colostrum on dyspepsia symptoms, intestinal permeability and faecal calprotectin as markers of gut injury. ANOVA of symptom scores showed that both colostrum and milk groups had a small but significant (p<O.Ol) reduction over time. However, there was no significant effect of treatment (p = 0.28). Initial intestinal permeability values were normal or only mildly elevated (0.07-0.80). ANOVA showed no significant effect of time (p = 0.66). Also initial faecal calprotectin levels of only 4 out of 12 subjects were elevated. Further analysis as to the effect of colostrum was not carried out because of incomplete stool collection by some of the subjects. Colostrum contains many factors that may have relevance for gut repair. The effect of nucleotides known to be present in colostrum and lipocalin 24p3, also present in colostrum, were studied using cell migration, proliferation assays and indomethacin gastric damage model. Nucleotide mixture caused a dose dependent increase in cell migration. Addition of individual nucleotides caused a significant increase in the rate of migration. Nucleotides had either no effect on cell proliferation or caused a growth inhibition. Subcutaneous administration of nucleotide mixture increased gastric damage. In contrast, oral administration of nu cl eo tides caused a dose related reduction in gastric injury. 24p3 caused cell migration in a dose dependent fashion but did not have an affect on cell proliferation. Administration of wild type 24p3 and mutant 24p3 (C98A) were both able to reduce the amount of gastric injury that occurred (both p < 0.01 vs. control).
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:582628 |
| Date | January 2007 |
| Creators | Belo, Angelica Chioma |
| Publisher | Imperial College London |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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