Mechanisms that result in directional tissue growth include cell proliferation, orientated cell division, an increase in cell size, a change in the cell density and cell death. These were investigated in the development of the mouse palatal shelves between stages E11.5 – E15.5. The aim was to develop and apply image processing tools to automatically analyse images of fixed specimens to reveal cellular behaviours that can be applied to understand directional growth and diagnosing the mechanism of its failure in mutant tissue in the palate and more generally. Techniques included immunohistochemistry after frozen or paraffin sectioning and imaging on the confocal microscope. Analysis of images was done using programs such as ImageJ, Volocity and R. This analysis was carried out on CD-1 wild-type mice to provide a thorough understanding of the mechanisms of cellular morphogenesis throughout normal palate development. It was then performed on mutant Tbx-1, Msx-1 and Wnt1-Cre;ERK2fl/fl mice all of which have a cleft palate phenotype to demonstrate how a better understanding of the links between genetic mutations, tissue phenotypes and cellular disruption can be gained.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:631313 |
| Date | January 2013 |
| Creators | Brock, Lara |
| Publisher | King's College London (University of London) |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | https://kclpure.kcl.ac.uk/portal/en/theses/cellular-mechanisms-of-morphogenesis-in-palate-development(775553e2-6cf0-4a62-be7c-372fbf43bf29).html |
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