Many studies have shown that mesenchymal stem cells (MSCs) can be used as immunosuppressants to treat graft-versus-host disease (GVHO) and autoimmune diseases. The injection of MSCs prolonged allogeneic solid organ transplantation in animal models. The current project aimed to investigate whether tissue-engineered cartilage derived from MSCs could be transplanted allogeneically. We mainly looked at murine MSCs (mMSCs) in this project. mMSCs are more difficult to isolate than human MSCs. We tried several methods to isolate mMSCs and found that a FACS isolated subpopulation of mMSCs, i.e. PaS cells (POGFRa+, Sca-1 +), had clear chondrogenic differentiation potential. The mechanisms involved in the immunosuppressive effect of MSCs are still under debate. Our in vitro data showed that the immunosuppressive effect of MSCs is through a negativefeedback loop. Pro-inflammatory cytokines from proliferating Iymphocytes activate MSCs to produce immunosuppressive molecules that suppress the proliferation of Iymphocytes. This immunosuppressive effect is shared with other stromal cells. A species difference exists in terms of the immunosuppressive effect of MSCs.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:633157 |
| Date | January 2014 |
| Creators | Zhang, Shang |
| Publisher | University of Bristol |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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