The design and synthesis of discontinuous epitopes of HIV-1 gp120 has been investigated. This has been exemplified by the design of a peptide to mimic a discontinuous but conserved region of gp120 containing residues important for CD4 binding. This peptide was synthesised using the Fmoc strategy of solid phase peptide synthesis. The problem of aspartimide formation as a side product in the synthesis was observed and this process was addressed in more detail. Polyclonal antisera were raised in mice against the purified peptide and the antipeptide antibodies characterised. Initial evidence suggests this peptide binds to CD4 and induces apoptosis.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:645024 |
| Date | January 1994 |
| Creators | Cotton, Graham J. |
| Publisher | University of Edinburgh |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://hdl.handle.net/1842/13477 |
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