Dying molecular hybridisation reactions with a cDNA Copy, the complexity of polysomal polyadenylated mRNA from the day-old chick lens was found to correspond to 5800-7200 sequences of average size, arranged in three abundance classes. Experiments with heterologous cDNA are consistent with the interpretation that essentially all the sequences expressed in eight-day embryonic neural retina and pigmented epithelium were found to be present in lens mRNA. A cDNA fraction complementary to the most abundant lens mRNAs (presumably the crystallin mRNAS), and representing a minimum of four sequences, was used to assay the dosage of putative crystallin sequences in other embryonic tissues. Neural retina and pigmented epithelium cytoplasmic mRNAs have low concentrations of these sequences, and they appear to be absent from mRNA prepared from headless bodies and muscle. Additionally, the sequences are amplified during appearance of crystallin-containing lentoid bodies in neural retina culture.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:652895 |
| Date | January 1977 |
| Creators | Jackson, James Franklin |
| Publisher | University of Edinburgh |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://hdl.handle.net/1842/15093 |
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