Enabling the entry of therapeutic antibodies into cells for the modulation of intracellular targets has the potential to greatly expand the treatment options for diseases, such as cancer. A class of promising delivery vehicles for therapeutic antibodies is the cell-penetrating peptides (CPP), which are capable of entering cells spontaneously and promote the cellular uptake of conjugated biomolecules, such as antibodies. Demonstration of the CPP-mediated delivery of a functional protein was performed using the well-established example of p27, a cyelin-dependent kinase inhibitor, fused to the Tat delivery domain of the human immunodeficiency virus (HIV). Concomitant with established data, the Tat-p27 fusion protein entered cells, induced G1 cell cycle arrest in MCF-7 cells and induced HepG2 cells to undergo cell scattering and cytoskeletal alterations.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:663236 |
| Date | January 2013 |
| Creators | Cheung, Jason W. C. |
| Publisher | University of Nottingham |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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