This project has focused on establishing a specific multipurpose labelling system via the modification of D-( + )-biotin in use with (strept)avidin proteins for purification and isolation. Two biotin analogues; 2-azidobiotin and 2-propargylbiotin, have been synthesised, introducing an alkyne and azide group at the 2 position of the valeryl side chain of biotin respectively. Isothermal titration calorimetry studies showed that the biotin analogues had a reduced affinity (Kd= 1V10-9 M) for strept(avidin) proteins, when compared to biotin (Kd= ~10-15 M).
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:664312 |
| Date | January 2014 |
| Creators | Sehmi, Inderpal Kaur |
| Publisher | University of Nottingham |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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