Chapter 1 describes deoxynivalenol and zearalenone mycotoxins as secondary metabolites of Fusarium fungi. Metabolism of Fusarium fungi is discussed in the light of deoxynivalenol and zearalenone biosynthesis. Consequences of the presence of toxic fungal metabolites in cereal products are highlighted. Chapter 2 focuses on production of material for degradation and binding studies i.e. gram quantities of both mycotoxins. Procedures of deoxynivalenol and zearalenone production by fermentation were successfully designed and executed. The identities of the compounds were confirmed by NMR. Additionally, radio labelled deoxynivalenol was produced using developed fermentation methods. Chapter 3 investigates degradation of deoxynivalenol and zearalenone under typical cereal processing conditions. Acidic and basic degradation models were developed and executed. Degradation products of deoxynivalenol and zearalenone were isolated and characterised by LC-MS and NMR. NMR time course experiments were successfully performed resulting in mechanistic and kinetic information of deoxynivalenol and zearalenone degradation. Chapter 4 presents studies on binding of deoxynivalenol to food components such starch and flour. Previously produced 14C-labelled deoxynivalenol served as a tracer during binding studies. Influence of extraction procedures on deoxynivalenol bound to starch was investigated. The existence of binding of deoxynivalenol to flour was observed. Speculations on the nature of binding were made.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:685430 |
| Date | January 2010 |
| Creators | Kowalczyk, Mateusz Jan |
| Publisher | University of Bristol |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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