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Phenotypic characterization of cartilage cells during endochondral ossification (an avian growth plate model).

by Lee Kwong Man, Simon. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 88-94. / ABSTRACT --- p.I / ACKNOWLEDGEMENTS --- p.IV / TABLE OF CONTENTS --- p.V / Chapter CHAPTER ONE - --- INTRODUCTION --- p.1 / Chapter CHAPTER TWO - --- STRUCTURE OF CARTILAGE / Chapter 2.1 --- Characteristics of Cartilage --- p.4 / Chapter 2.2 --- Types of Cartilage --- p.4 / Chapter 2.3 --- Matrix --- p.5 / Chapter 2.3.1 --- Collagen --- p.5 / Chapter 2.3.2 --- Proteoglycan --- p.7 / Chapter 2.4 --- Diffusion of Solultes in Cartilage --- p.9 / Chapter 2.5 --- Chondroytes --- p.10 / Chapter CHAPTER THREE - --- PHYSIOLOGICAL CHANGES WITHIN CARTILAGE / Chapter 3.1 --- Endochondral Ossification --- p.12 / Chapter 3.2 --- Hormone Responses on Cartilage --- p.16 / Chapter 3.3 --- Degradative enzymatic system --- p.17 / Chapter 3.3.1 --- Evidence for the Role of Enzymes in osteoarthritis --- p.18 / Chapter 3.3.2 --- Neutral Protease Acivity --- p.20 / Chapter 3.3.3 --- Neutal proteases in osteoarthritis --- p.21 / Chapter 3.3.4 --- Collagenase activity in articular cartilage --- p.22 / Chapter CHAPTER FOUR - --- METHODOLOGIES / Chapter 4.1 --- Isolation of Chick Growth Plate and Articular Chondrocytes --- p.24 / Chapter 4.2 --- Countercurrent Centrifugal Elutriation --- p.25 / Chapter 4.3 --- Size Determination of Chondrocytes --- p.26 / Chapter 4.4 --- Chondrocyte Cell Culture --- p.28 / Chapter 4.5 --- Flow Cytometry Cell Cycle Analysis of Elutriated Chondrocytes --- p.28 / Chapter 4.6 --- Thymidine Incorporation Assay on Elutriated Chondrocytes --- p.29 / Chapter 4.7 --- Sulfur Incorporation Assay on Elutriated Chondrocytes --- p.30 / Chapter 4.8 --- Hyalurondiase Assay on Elutriated Chondrocytes --- p.31 / Chapter 4.9 --- Alkaline Phosphatase Assay on Elutriated Chondrocytes --- p.32 / Chapter 4.10 --- Acid Phosphatase Assay --- p.33 / Chapter 4.10.1 --- Total Acid Phosphatase Assay on Elutriated Chondrocytes --- p.33 / Chapter 4.10.2 --- Optimal pH Determination of Phosphatase in Isolated Chondrocytes --- p.33 / Chapter 4.10.3 --- Enzyme Kinetics of Acid Phosphatase of Isolated Growth Plate and Articular Chondrocytes --- p.34 / Chapter 4.10.4 --- Tartrate Inhibition Effect on Acid Phosphatase of Growth Plate and Articular Chondrocytes --- p.35 / Chapter 4.10.5 --- Distribution of Acid Phosphatase Isoenzymes Among Chondrocytes of Different Size --- p.35 / Chapter 4.11 --- Hormonal Effects on Acid and Alkaline Phosphatase Activities in Growth Plate and Articular Chondrocytes --- p.36 / Chapter CHAPTER FIVE - --- RESULTS / Chapter 5.1 --- Morphology of the Isolated Chick Chondrocytes --- p.39 / Chapter 5.2 --- Countercurrent Centrifugal Elutriation VI --- p.39 / Chapter 5.3 --- Thymidine Incorporation Assay on Elutriated Chondrocytes --- p.44 / Chapter 5.4 --- Flow Cytometer Cell Cycle Analysis of Elutriated Chondrocytes --- p.44 / Chapter 5.5 --- Sulfate Incorporation Assay on Elutriated Chondrocytes --- p.48 / Chapter 5.6 --- Hyaluronidase Assay on Elutriated Chondrocytes on --- p.48 / Chapter 5.7 --- Alkaline Phosphatase Assay on Elutriaed Chondrocytes --- p.48 / Chapter 5.8 --- Acid Phosphatase Assay --- p.52 / Chapter 5.8.1 --- pH Curve of Phosphatase in Isolated Chondrocytes --- p.52 / Chapter 5.8.2 --- Enzyme Kinetics of Acid Phosphatase oflsolated Growth Plate and Articular Chondrocytes --- p.52 / Chapter 5.8.3 --- Tartrate Inhibition Effect on Acid Phosphatae of Growth Plate and Articular Chondrocytes --- p.55 / Chapter 5.8.4 --- Distribution of Acid Phosphatase Isoenzymes Among Chondrocytes in Different Size --- p.57 / Chapter 5.9 --- Hormonal Effects on Acid and Alkaline Phosphatase Activities in Growth Plate and Articular Chondrocytes --- p.59 / Chapter CHAPTER SIX - --- DISCUSSION / Chapter 6.1 --- Identification of Chondrocyte Subpopulations --- p.63 / Chapter 6.2 --- Characterization of Chondrocyte Subpopulations --- p.72 / Chapter 6.3 --- Characterization of Acid Phosphatase in Chick Chondrocytes --- p.74 / LIST OF FIGURES --- p.84 / LIST OF TABLES --- p.87 / REFERENCES --- p.88 / Chapter APPENDIX I --- Principle of Countercurrent Centrifugal Elutriation --- p.95 / Chapter APPENDIX II --- Principle of Flow Cytometry --- p.98 / Chapter APPENDIX III --- Reagents for Experiments --- p.103

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318592
Date January 1990
ContributorsLee, Kwong Man, Simon., Chinese University of Hong Kong Graduate School. Division of Clinical and Pathological Sciences.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, viii, 105 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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