by Lee Kwong Man, Simon. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 88-94. / ABSTRACT --- p.I / ACKNOWLEDGEMENTS --- p.IV / TABLE OF CONTENTS --- p.V / Chapter CHAPTER ONE - --- INTRODUCTION --- p.1 / Chapter CHAPTER TWO - --- STRUCTURE OF CARTILAGE / Chapter 2.1 --- Characteristics of Cartilage --- p.4 / Chapter 2.2 --- Types of Cartilage --- p.4 / Chapter 2.3 --- Matrix --- p.5 / Chapter 2.3.1 --- Collagen --- p.5 / Chapter 2.3.2 --- Proteoglycan --- p.7 / Chapter 2.4 --- Diffusion of Solultes in Cartilage --- p.9 / Chapter 2.5 --- Chondroytes --- p.10 / Chapter CHAPTER THREE - --- PHYSIOLOGICAL CHANGES WITHIN CARTILAGE / Chapter 3.1 --- Endochondral Ossification --- p.12 / Chapter 3.2 --- Hormone Responses on Cartilage --- p.16 / Chapter 3.3 --- Degradative enzymatic system --- p.17 / Chapter 3.3.1 --- Evidence for the Role of Enzymes in osteoarthritis --- p.18 / Chapter 3.3.2 --- Neutral Protease Acivity --- p.20 / Chapter 3.3.3 --- Neutal proteases in osteoarthritis --- p.21 / Chapter 3.3.4 --- Collagenase activity in articular cartilage --- p.22 / Chapter CHAPTER FOUR - --- METHODOLOGIES / Chapter 4.1 --- Isolation of Chick Growth Plate and Articular Chondrocytes --- p.24 / Chapter 4.2 --- Countercurrent Centrifugal Elutriation --- p.25 / Chapter 4.3 --- Size Determination of Chondrocytes --- p.26 / Chapter 4.4 --- Chondrocyte Cell Culture --- p.28 / Chapter 4.5 --- Flow Cytometry Cell Cycle Analysis of Elutriated Chondrocytes --- p.28 / Chapter 4.6 --- Thymidine Incorporation Assay on Elutriated Chondrocytes --- p.29 / Chapter 4.7 --- Sulfur Incorporation Assay on Elutriated Chondrocytes --- p.30 / Chapter 4.8 --- Hyalurondiase Assay on Elutriated Chondrocytes --- p.31 / Chapter 4.9 --- Alkaline Phosphatase Assay on Elutriated Chondrocytes --- p.32 / Chapter 4.10 --- Acid Phosphatase Assay --- p.33 / Chapter 4.10.1 --- Total Acid Phosphatase Assay on Elutriated Chondrocytes --- p.33 / Chapter 4.10.2 --- Optimal pH Determination of Phosphatase in Isolated Chondrocytes --- p.33 / Chapter 4.10.3 --- Enzyme Kinetics of Acid Phosphatase of Isolated Growth Plate and Articular Chondrocytes --- p.34 / Chapter 4.10.4 --- Tartrate Inhibition Effect on Acid Phosphatase of Growth Plate and Articular Chondrocytes --- p.35 / Chapter 4.10.5 --- Distribution of Acid Phosphatase Isoenzymes Among Chondrocytes of Different Size --- p.35 / Chapter 4.11 --- Hormonal Effects on Acid and Alkaline Phosphatase Activities in Growth Plate and Articular Chondrocytes --- p.36 / Chapter CHAPTER FIVE - --- RESULTS / Chapter 5.1 --- Morphology of the Isolated Chick Chondrocytes --- p.39 / Chapter 5.2 --- Countercurrent Centrifugal Elutriation VI --- p.39 / Chapter 5.3 --- Thymidine Incorporation Assay on Elutriated Chondrocytes --- p.44 / Chapter 5.4 --- Flow Cytometer Cell Cycle Analysis of Elutriated Chondrocytes --- p.44 / Chapter 5.5 --- Sulfate Incorporation Assay on Elutriated Chondrocytes --- p.48 / Chapter 5.6 --- Hyaluronidase Assay on Elutriated Chondrocytes on --- p.48 / Chapter 5.7 --- Alkaline Phosphatase Assay on Elutriaed Chondrocytes --- p.48 / Chapter 5.8 --- Acid Phosphatase Assay --- p.52 / Chapter 5.8.1 --- pH Curve of Phosphatase in Isolated Chondrocytes --- p.52 / Chapter 5.8.2 --- Enzyme Kinetics of Acid Phosphatase oflsolated Growth Plate and Articular Chondrocytes --- p.52 / Chapter 5.8.3 --- Tartrate Inhibition Effect on Acid Phosphatae of Growth Plate and Articular Chondrocytes --- p.55 / Chapter 5.8.4 --- Distribution of Acid Phosphatase Isoenzymes Among Chondrocytes in Different Size --- p.57 / Chapter 5.9 --- Hormonal Effects on Acid and Alkaline Phosphatase Activities in Growth Plate and Articular Chondrocytes --- p.59 / Chapter CHAPTER SIX - --- DISCUSSION / Chapter 6.1 --- Identification of Chondrocyte Subpopulations --- p.63 / Chapter 6.2 --- Characterization of Chondrocyte Subpopulations --- p.72 / Chapter 6.3 --- Characterization of Acid Phosphatase in Chick Chondrocytes --- p.74 / LIST OF FIGURES --- p.84 / LIST OF TABLES --- p.87 / REFERENCES --- p.88 / Chapter APPENDIX I --- Principle of Countercurrent Centrifugal Elutriation --- p.95 / Chapter APPENDIX II --- Principle of Flow Cytometry --- p.98 / Chapter APPENDIX III --- Reagents for Experiments --- p.103
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318592 |
| Date | January 1990 |
| Contributors | Lee, Kwong Man, Simon., Chinese University of Hong Kong Graduate School. Division of Clinical and Pathological Sciences. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, viii, 105 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
Page generated in 0.0026 seconds