by Hon-chiu Leung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1991. / Bibliography: leaves 122-126. / Abstract --- p.i / Acknowledgement --- p.iv / Dedication --- p.v / Table of Contents --- p.vi / Introduction --- p.1 / Literature Review --- p.4 / Chapter 1) --- Ethanol production in bacteria / Chapter 1.1) --- Zymomonas mobilis --- p.4 / Chapter 1.2) --- Clostridium species --- p.7 / Chapter 1.3) --- "Enterobacter, Klebsiella, Serritia and Erwinia sp" --- p.9 / Chapter 1.4) --- Escherichia coli and Salmonella typhimurium --- p.10 / Chapter 2) --- Pyruvate decarboxylase of Z. mobilis / Chapter 2.1) --- Enzyme properties --- p.13 / Chapter 2.2) --- Cloning and expression of pdc gene --- p.15 / Chapter 3) --- Alcohol dehydrogenase (adh) gene / Chapter 3.1) --- "Cloning, chararterization and expression of adh genes" --- p.17 / Chapter 4) --- Gene transfer systems in Vibrio species --- p.21 / Chapter 5) --- Rationale and objectives of this study --- p.22 / Chapter Part I) --- Ethanol Production in terrestrial enteric bacteria / Chapter A) --- Introduction --- p.24 / Chapter B) --- Materials and Methods / Chapter 1) --- Bacterial strains and plasmids --- p.25 / Chapter 2) --- Media --- p.26 / Chapter 3) --- Solutions --- p.27 / Chapter 4) --- Isolation of plasmids / Chapter 4.1) --- Small Scale Isolation of plasmids --- p.30 / Chapter 4.2) --- Large Scale Isolation of plasmids --- p.32 / Chapter 5) --- Construction of a broad-host-range plasmid harbouring Zymomonas mobilis genes --- p.35 / Chapter 6) --- Transformation --- p.35 / Chapter 7) --- High Performance Liquid Chromatography of Organic Acids --- p.36 / Chapter 8) --- Maintenace of plasmids harbouring the genes of Zymomonas mobilis genes --- p.38 / Chapter 9) --- Ethanol tolerance of S. typhimurium strains --- p.38 / Chapter C) --- Results / Chapter 1) --- Construction of Salmonella typhimurium strains harbouring Z. mobilis genes --- p.39 / Chapter 2) --- Fermentative end products in culture medium --- p.48 / Chapter 3) --- Growth of hosts and transformants --- p.61 / Chapter 4) --- Ethanol tolerance of S. typhimurium strains --- p.65 / Chapter 5) --- Maintenance of plasmids --- p.67 / Chapter 6) --- Construction of broad-host-range plasmid harbouring Z. mobilis genes --- p.69 / Chapter D) --- Discussions / Chapter 1) --- Comparison of ethanol production in Escherichia coli and Salmonella typhimurium --- p.72 / Chapter 2) --- Ethanol tolerance of S. typhimurium strains --- p.74 / Chapter 3) --- Maintenance of plasmids --- p.76 / Chapter 4) --- Construction of broad-host-range plasmids harbouring Z. mobilis genes --- p.78 / Chapter Part II) --- Ethanol Production in marine enteric bacteria / Chapter A) --- Introduction --- p.79 / Chapter B) --- Materials and Methods / Chapter 1) --- Bacterial strains and plasmids --- p.80 / Chapter 2) --- Media --- p.80 / Chapter 3) --- Solutions --- p.80 / Chapter 4) --- Routine Identification Processes --- p.81 / Chapter 5) --- Systematic studies by Arbitrarily- Primed Polymerase Chain Reaction --- p.86 / Chapter 6) --- Optimal growth conditions --- p.88 / Chapter 7) --- Isolation of broad-host-range plasmid pIOl ( 64kb) --- p.89 / Chapter 8) --- Transformation of Vibrio sp. strain 60 --- p.90 / Chapter 9) --- Production of ethanol using different carbon sources in fermentation --- p.91 / Chapter C) --- Results / Chapter 1) --- Identification of Vibrio sp. strain 60 --- p.92 / Chapter 2) --- Optimal growth conditions --- p.101 / Chapter 3) --- Isolation of high molecular weight plasmid --- p.105 / Chapter 4) --- Ethanol production from different carbon sources --- p.107 / Chapter 5) --- Ethanol tolerance of Vibrio sp. strain 60 --- p.109 / Chapter 6) --- Salt tolerance of Vibrio sp. strain 60 --- p.111 / Chapter 7) --- Transformation of Vibrio sp. strain 60 --- p.113 / Chapter D) --- Discussions / Chapter 1) --- Strain identification by arbitrarily-primed PCR --- p.116 / Chapter 2) --- Isolation of high molecular weight plasmid --- p.118 / Chapter 3) --- Ethanol production of Vibrio sp. strain60 --- p.120 / References --- p.122
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318727 |
| Date | January 1991 |
| Contributors | Leung, Hon-chiu., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, x, 126 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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