by Chan Eliza. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 90-95). / ACKNOWLEDGEMENTS --- p.i / ABSTRACT --- p.ii / Chapter CHAPTER 1 --- INTRODUCTION --- p.1 / Chapter CHAPTER 2 --- LITERATURE REVIEW / Chapter SECTION 1 --- Classification of cutaneous mechanoreceptors in the mammalian skin --- p.3 / Chapter 1.1 --- Criteria --- p.3 / Chapter 1.2 --- Slowly adapting type I mechanoreceptors --- p.3 / Chapter 1.3 --- Rapidly adapting mechanoreceptors --- p.6 / Chapter SECTION 2 --- Structural features of Merkel cells --- p.8 / Chapter 2.1 --- History --- p.8 / Chapter 2.2 --- General morphology of Merkel cells --- p.8 / Chapter 2.3 --- Electron microscopical description of the Merkel cell-neurite complexes --- p.10 / Chapter SECTION 3 --- Responsive features of Merkel cell as slowly adapting type I mechanoreceptors --- p.12 / Chapter 3.1 --- History --- p.12 / Chapter 3.2 --- Principles of the in-vivo and in-vitro techniques --- p.13 / Chapter 3.2.1 --- Location of Merkel cells --- p.13 / Chapter 3.2.2 --- Characteristic firing pattern of the slowly adapting type I mechanoreceptor --- p.14 / Chapter SECTION 4 --- Functional implications of the Merkel cell --- p.18 / Chapter 4.1 --- An analogy between Merkel cells and sensory hair cells of the auditory system --- p.18 / Chapter 4.1.1 --- Sensory hair cells of the acoustico-lateralis system --- p.18 / Chapter 4.1.2 --- Mechano-electrical activity of the Merkel cells --- p.21 / Chapter 4.2 --- Existence of dense-core vesicles --- p.21 / Chapter 4.2.1 --- Hypoxia reduced excitability of slowly adapting type I mechanoreceptors --- p.23 / Chapter 4.2.2 --- Calcium blockers affect the responsiveness of the slowly adapting type I mechanoreceptors --- p.24 / Chapter 4.3 --- Met-enkephalin as a putative neurotransmitter --- p.25 / Chapter SECTION 5 --- Met-enkephalin as an endogenous opioid peptide --- p.26 / Chapter 5.1 --- Synthesis and metabolic regulation of met-enkephalin --- p.26 / Chapter 5.2 --- The opioid receptors --- p.27 / Chapter 5.3 --- "Selective μ-, δ- and kappa- opioid receptor antagonists" --- p.28 / Chapter CHAPTER 3 --- METHODS / Chapter SECTION 1 --- In-vitro study --- p.30 / Chapter 1.1 --- Dissection --- p.30 / Chapter 1.2 --- Identification of a receptor and administration of chemicals --- p.34 / Chapter 1.2.1 --- Firing patterns of the type I and type II mechanoreceptors --- p.35 / Chapter 1.2.2 --- Interspike interval distributions (ISI) --- p.37 / Chapter 1.3 --- Administration of drugs --- p.39 / Chapter SECTION 2 --- Experimental setup --- p.39 / Chapter 2.1 --- Mechanical stimulation --- p.39 / Chapter 2.2 --- Recordings --- p.40 / Chapter 2.3 --- Data processing --- p.41 / Chapter SECTION 3 --- Preparation of drugs --- p.43 / Chapter 3.1 --- Mu- opioid receptor antagonists --- p.43 / Chapter 3.2 --- Delta- opioid receptor antagonist --- p.43 / Chapter 3.3 --- Kappa- opioid receptor antagonists --- p.44 / Chapter SECTION 4 --- Data analysis --- p.44 / Chapter 4.1 --- Comparison of data --- p.44 / Chapter 4.2 --- Statistics --- p.45 / Chapter CHAPTER 4 --- RESULTS / Chapter SECTION 1 --- Determination of an optimal stimulation force --- p.46 / Chapter SECTION 2 --- Effects of the mu- opioid receptor antagonists --- p.48 / Chapter 2.1 --- Naloxone --- p.48 / Chapter 2.2 --- β-FNA --- p.54 / Chapter 2.2.1 --- Slowly adapting type I mechanoreceptor --- p.54 / Chapter 2.2.2 --- Control study of vehicle --- p.54 / Chapter SECTION 3 --- Effects of the delta- opioid receptor antagonist ICI174864 --- p.58 / Chapter SECTION 4 --- Effects of the kappa- opioid receptor antagonists --- p.58 / Chapter 4.1 --- nor-BNI --- p.58 / Chapter 4.1.1 --- Slowly adapting type I mechanoreceptor --- p.58 / Chapter 4.1.2 --- Afferent nerve attached to the type I mechanoreceptor --- p.62 / Chapter 4.2 --- MR2266 --- p.65 / Chapter 4.2.1 --- Control study of vehicle --- p.67 / Chapter 4.2.2 --- Slowly adapting type I mechanoreceptor --- p.67 / Chapter 4.2.3 --- Afferent nerve attached to the type I mechanoreceptor --- p.73 / Chapter CHAPTER 5 --- DISCUSSION AND CONCLUSION / Chapter SECTION 1 --- Study of the slowly adapting type I mechanoreceptors using the in-vitro preparation --- p.80 / Chapter 1.1 --- Characteristic features of the slowly adapting type I mechanoreceptor --- p.81 / Chapter 1.2 --- Optimal force of stimulation --- p.82 / Chapter SECTION 2 --- Effects of the opioid receptor antagonists --- p.82 / Chapter 2.1 --- Lack of effects of the μ- and δ- opioid receptor antagonists --- p.83 / Chapter 2.2 --- The kappa- opioid receptor antagonists --- p.85 / Chapter 2.2.1 --- nor-BNI --- p.85 / Chapter 2.2.2 --- MR2266 --- p.86 / Chapter SECTION 3 --- Existence of opioidergic receptor sites in the Merkel cell-neurite complexes ？ --- p.87 / REFERENCES --- p.90
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318909 |
| Date | January 1992 |
| Contributors | Chan, Eliza., Chinese University of Hong Kong Graduate School. Division of Basic Medical Sciences. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, iii, 95 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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