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Preservation of blood glucose.

Leung Kwok-ming, Victor. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 74-78). / Chapter 1. --- SUMMARY --- p.1 / Chapter 2. --- INTRODUCTION / Chapter 2.1 --- Glycolysis : magnitude of the problem --- p.2 / Chapter 2.2 --- Glycolysis : individual variations --- p.2 / Chapter 2.2.1 --- Erythrocyte --- p.2 / Chapter 2.2.2 --- Leucocyte --- p.3 / Chapter 2.2.3 --- Age of individual --- p.3 / Chapter 2.3 --- Methods of blood glucose preservation --- p.3 / Chapter 2.3.1 --- Sodium fluoride --- p.4 / Chapter 2.3.1.1 --- Delayed effectiveness of NaF ; clinical implications --- p.6 / Chapter 2.3.2 --- D-mannose --- p.7 / Chapter 2.3.3 --- Citric acid --- p.9 / Chapter 2.3.4 --- Cooling --- p.10 / Chapter 2.3.5 --- Haemolysis --- p.11 / Chapter 2.4 --- Aims of the project --- p.12 / Chapter 3. --- MATERIALS & METHODS / Chapter 3.1 --- Evaluation of the YSI 23 AM glucose analyser for the measurement of glucose in haemolysate --- p.15 / Chapter 3.1.1 --- Principle of measurement --- p.15 / Chapter 3.1.2 --- Preparation of haemolysate --- p.16 / Chapter 3.1.3 --- Procedure --- p.16 / Chapter 3.2 --- Development of a glucose assay in haemolysate in Cobas Bio and Cobas Mira --- p.17 / Chapter 3.2.1 --- Glucose measurement --- p.17 / Chapter 3.2.2 --- Preparation of haemolysate --- p.17 / Chapter 3.2.3 --- Cobas Bio --- p.17 / Chapter 3.2.3.1 --- Optimisation of sample size --- p.17 / Chapter 3.2.3.2 --- Discussion --- p.21 / Chapter 3.2.4 --- Cobas Mira --- p.22 / Chapter 3.2.4.1 --- Optimisation of sample/reagent volume ratio --- p.22 / Chapter 3.2.4.2 --- Discussion --- p.22 / Chapter 3.2.4.3 --- Optimisation of the assay protocol --- p.26 / Chapter 3.2.4.4 --- Evaluation of the RS-SR1 protocol --- p.34 / Chapter 3.2.4.5 --- Discussion --- p.36 / Chapter 3.2.5 --- To study the effect of variations in haematological parameters on the re- covery of glucose inhaemolysate --- p.36 / Chapter 3.2.5.1 --- Procedure / :Experiment (i) --- p.36 / :Experiment (ii) --- p.43 / Chapter 3.2.5.2 --- Discussion --- p.48 / Chapter 3.2.6 --- Interference study of saponin --- p.50 / Chapter 3.2.6.1 --- Procedure --- p.50 / Chapter 3.2.6.2 --- Discussion --- p.50 / Chapter 3.2.7 --- Interference study of SDS --- p.53 / Chapter 3.2.7.1 --- Procedure --- p.53 / Chapter 3.2.7.2 --- Discussion --- p.53 / Chapter 3.3 --- Stability of glucose in haemolysate --- p.55 / Chapter 3.3.1 --- Preparation of haemolysate --- p.55 / Chapter 3.3.2 --- Glucose measurement --- p.55 / Chapter 3.3.3 --- Procedure --- p.55 / Chapter 3.3.3.1 --- Experiment (i) --- p.55 / Chapter 3.3.3.2 --- Experiment (ii) --- p.55 / Chapter 3.3.4 --- Statistical analysis --- p.56 / Chapter 4. --- RESULTS / Chapter 4.1 --- Evaluation of the YSI 23 AM glucose analyser for the measurement of glucose in haemolysate --- p.57 / Chapter 4.2 --- Stability of glucose in haemolysate --- p.60 / Chapter 5. --- DISCUSSION --- p.66 / Chapter 6. --- ACKNOWLEDGMENT --- p.73 / Chapter 7. --- REFERENCES --- p.74

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318913
Date January 1992
ContributorsLeung, Kwok-ming., Chinese University of Hong Kong Graduate School. Division of Clinical and Pathological Sciences.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, 78 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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